Venn diagrams, showing DNA shared and unique among isolates. Venn diagrams showing the unique genomic material in the isolates when partitioned by species, host and region. All regions were identified by finding the core genome of the isolates within the set and removing the pan-genome of all isolates excluded from the set. The unique material for each partition is shown as total size in base pairs, number of segments in partition with a minimum size of 200 bp, the length of the largest segment and the number of CDS with an overlap of >10%. The intersection of the sets was determined by finding the core genome of the union of the sets of isolates and shows the size of the core and the number of CDS with an overlap of >10%. (A) Shows the partition between all 30 M. ulcerans isolates and all four M. marinum isolates. (B) Shows the partition between the four M. ulcerans fish and frog host isolates and the M. ulcerans human host isolates. The human host isolates are a strict subset of the fish and frog isolates with no unique DNA. (C) Shows the partitioning between the 10 Australian and 13 African M. ulcerans isolates and their separation from the isolates from the rest of the world (6 isolates). The combined African and Australian isolates have no unique DNA when compared to the rest of the world isolates.