Figure 2
From: Origin of a novel protein-coding gene family with similar signal sequence in Schistosoma japonicum
Southern blotting confirms duplicated loci exclusively in S. japonicum. (A) Southern hybridization with digoxigenin-labeled probes showing the presence of duplicated loci with several bands due to copies of the duplicated source locus. Lanes 2-9 corresponds to Eco RI + Eco RV double digested genomic DNA of different species and strains of Schistosoma (S. haematobium, S. mansoni, S. mekongi, S. japonicum (Japanese, Chinese, Philippines’ Leyte, Mindanao and Mindoro isolates). ‘M’ is Digoxigenin-labeled DNA molecular weight marker. Notice the differential banding pattern among different strains and between isolates of the same strain. (B) Same experiment as in (A) was replicated using a different pair of restriction enzymes (EcoRI + Hind III). Also inter-strain and intra-strain variation in the banding pattern is apparent.