Skip to main content

Table 2 Twenty-one yeast overexpression strains confirmed as suppresors of sensitivity to COS-5.44

From: Identification of yeast genes that confer resistance to chitosan oligosaccharide (COS) using chemogenomics

ORF Gene Resistance confirmed Fitness score of resistance (avg)3 Sensitivity confirmed (HET deletion strain) Fitness score of sensitivity (avg)4 Description from SGD
YBR164C ARL11 yes 1.870 yes 0.756 Soluble GTPase of the Ras superfamily, with a role in regulation of membrane traffic mainly regulates potassium influx; similar to ADP-ribosylation factor.
YBR166C TYR1 yes 1.381 no 1.035 Prephenate dehydrogenase involved in tyrosine biosynthesis, expression is dependent on phenylalanine levels.
YDL029W ARP2 yes 1.609 no 1.023 Essential component of the Arp2/3 complex involved in endocytosis and membrane growth and polarity. A conserved actin nucleation center required for the motility and integrity of actin patches.
YDR171W HSP42 yes 1.372 no 1.135 Small heat shock protein (sHSP) with chaperone activity involved in cytoskeleton reorganization after heat shock; forms barrel-shaped oligomers that suppress unfolded protein aggregation.
YDR524C AGE1 yes 1.783 no 1.106 ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways.
YDR527W RBA501 yes 1.407 yes 0.899 Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1.
YER048C CAJ1 yes 1.845 no 0.995 Nuclear type II J heat shock protein of the of the E. coli dnaJ family, binds, to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly.
YER167W BCK21 yes 1.365 yes 0.890 Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc 1 mutations
YJL046W AIM22 yes 1.258 no 0.908 Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p.
YKL208W CBT1 yes 1.265 no 0.930 a role in 3′end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase.
YLR193C UPS1 yes 1.201 no 0.953 Mitochondrial intermembrane space protein that regulates mitochondrial cardiolipin levels, null has defects in Mgm1p processing, integrity of mitochondrial inner membrane complexes; ortholog of human PRELI.
YLR285W NNT1 yes 1.448 no 1.184 Putative nicotinamide N-methyltransferase, has a role in rDNA silencing and in the lifespan determination.
YML124C TUB3 yes 1.763 no 1.227 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p.
YMR123W PKR12 yes 1.350 yes 0.866 V-ATPase assembly factor, functions with other V-ATPase assembly factors in the ER to efficiently assemble the VATPase membrane sector (VO).
YNL053W MSG51 yes 1.454 yes 0.874 Dual-specificity protein phosphatase; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p.
YNL054W VAC72 yes 1.387 yes 0.873 Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activities Fab1p kinase activity under basal conditions anfd also after hyperosmotic shock.
YNL218W MSG12 yes 1.214 yes 0.899 Protein with DNA-dependent ATPase and ssDNA annealing activities involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human WHIP.
YNL280C ERG241 yes 1.275 yes 0.893 C-14 sterol reductase, acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8, 14 dienol).
YNR057C BIO42 yes 1.246 yes 0.885 Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis.
YPL053C KTR62 yes 1.616 yes 0.861 Probable mannosylphosphate transferase involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family.
YPL106C SSE12 yes 1.253 yes 0.896 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm.
  1. 1 Genes selected for transcriptomic analysis.
  2. 2 Overexpressing strains confirmed as suppressors when overexpressed and sensitive when tested as deletion strains.
  3. 3–4 Fitness score (W) is estimated from the average growth curves (as measured by optical density) of a given strain in the presence and absence of COS after 20 h. Data shown corresponds to deletion strains exposed to 91.1 μg/ml and overexpressing strains to 112.5 μg/ml of chitosan oligosaccharide. The fitness scores from three individual colonies of each overexpressing or deletion strain grown in triplicate (i.e. 9 cultures for each strain) were used to calculate the average. This score takes into account the growth of the wild type and overexpressing or deletion strain in control conditions as well as treatment conditions. W c  = wtc/stc; Wt = wtt/stt, where W c is the fitness score under control (no drug) conditions, W t is the fitness score after treatment, wt is doubling time of wild type strain, st is doubling time of the overexpressing or deletion strain, c is control conditions, t is treatment (COS) conditions. Normalized fitness (W) = avg Wt/avg Wc[24]. A fitness score less than 1 indicates that the strain is sensitive, a fitness score above 1 indicates that the strain is resistant [24]. Means that the strain has a treatment induced growth defect, a minimum difference of 0.1 (10%) was used as a cut-off to consider a strain sensitive.