Gene expression and proteomic analysis of the formation of Phakopsora pachyrhizi appressoria

Stone, Christine L; McMahon, Michael B; Fortis, Laurie L; Nuñez, Alberto; Smythers, Gary W; Luster, Douglas G; Frederick, Reid D

doi:10.1186/1471-2164-13-269

BMC Genomics

Table 3 Putative proteins identified during Phakopsora pachyrhizi appressoria formation grouped into functional categories using the Munich Information Center of Protein Sequences (MIPS) Functional Catalogue

From: Gene expression and proteomic analysis of the formation of Phakopsora pachyrhizi appressoria

Protein ID¹	GeneInfo Identifier No.	Description	Species	Peptides	Score
01. Metabolism
PHAP0002	gi\|254121616	2-isopropylmalate synthase	Melampsora larici-populina	4	151
PHAP0008	gi\|120528021	5-methyltetrahydropteroyl	Phakopsora pachyrhizi	3	116
		triglutamate-homocysteine
		methyltransferase
PHAP0010	gi\|120509127	acetyl-CoA C-acyltransferase	P. pachyrhizi	11	649
PHAP0031	gi\|120522633	deoxyuridine 5'-triphosphate	P. pachyrhizi	6	416
		nucleotidohydrolase
PHAP0032	gi\|120523312	dienelactone hydrolase	P. pachyrhizi	10	243
PHAP0043	gi\|120500948	glycine dehydrogenase	P. pachyrhizi	3	115
PHAP0064	gi\|120519389	isocitrate dehydrogenase	P. pachyrhizi	5	102
PHAP0065	gi\|120515452	isocitrate lyase	P. pachyrhizi	4	147
PHAP0071	gi\|290897165	NADP-dependent mannitol	M. larici-populina	3	83
		dehydrogenase
PHAP0103	gi\|90563667	methylene-tetrahydrofolate	Leucosporidium scottii	3	89
		dehrogenase
PHAP0109	gi\|254151530	UTP-glucose-1-phosphate	M. larici-populina	7	129
		uridylyltransferase
02. Energy
PHAP0027	gi\|120521145	cytochrome C	P. pachyrhizi	10	428
PHAP0089	gi\|120515891	pyruvate dehydrogenase beta	P. pachyrhizi	12	339
PHAP0101	gi\|120500717	succinate-CoA ligase beta	P. pachyrhizi	6	171
PHAP0107	gi\|161646983	ubiquinol-cytochrome C	Sporobolomyces roseus	6	78
		reductase
10. Cell cycle and DNA processing
PHAP0024	gi\|290902725	cell division cycle protein	Melampsora medusae	8	214
		cdc48	f. sp. deltoidis
PHAP0044	gi\|118187570	GTP binding protein/GTPase	Ustilago maydis	5	91
PHAP0074	gi\|254164953	nuclear segregation protein	M. larici-populina	3	66
PHAP0076	gi\|66651924	nucleoside diphosphate kinase	Uromyces viciae-fabae	5	173
PHAP0096	gi\|120423194	septin	Uromyces appendiculatus	1	52
11. Transcription
PHAP0084	gi\|120503605	polyadenylate binding protein	P. pachyrhizi	9	379
12. Protein Synthesis
PHAP0117²	gi\|120521780	40 S ribosomal protein S16	P. pachyrhizi	5	89
PHAP0006	gi\|282815640	40 S ribosomal protein S19	Puccinia tricina	10	368
PHAP0118²	gi\|120498576	60 S acidic ribosomal protein	P. pachyrhizi	5	88
PHAP0009	gi\|120522205	60 S ribosomal protein L10	P. pachyrhizi	13	732
14. Protein Fate (folding, modification, destination)
PHAP0026	gi\|169738476	cyclophilin	U. appendiculatus	6	118
PHAP0038³	gi\|120515849	glucose-regulated protein	P. pachyrhizi	21	379
PHAP0050	gi\|290908126	hsp-10 protein	Melampsora occidentalis	6	198
PHAP0073³	gi\|120425527	neddylin/ubiquitin	U. appendiculatus	3	129
PHAP0087	gi\|120523430	proteasome subunit beta	P. pachyrhizi	9	343
PHAP0113³	gi\|120515648	vacuolar protease A	P. pachyrhizi	7	153
16. Structural & Protein Binding
PHAP0013	gi\|120527115	actin lateral binding protein	P. pachyrhizi	19	494
18. Regulation
PHAP0097	gi\|156597317	serine-threonine phosphatase	Microbotryum violaceum	3	60
PHAP0099	gi\|120527960	sterol binding protein	P. pachyrhizi	11	113
20. Cellular transport, transport facilitation and transport routes
PHAP0120²	gi\|120511797	ADP, ATP carrier protein	P. pachyrhizi	6	96
PHAP0033	gi\|120523715	electron transport flavoprotein	P. pachyrhizi	5	200
		alpha
PHAP0067	gi\|120512188	mitochondrial import receptor	P. pachyrhizi	9	423
		TOM40
PHAP0072	gi\|169735819	nascent polypeptide-associated	U. appendiculatus	2	109
		complex subunit alpha
PHAP0100	gi\|254113321	succinate dehydrogenase	M. larici-populina	8	302
32. Cell Rescue, Defense and Virulence
PHAP0029	gi\|118188717	cytochrome C peroxidase	U. maydis	6	195
PHAP0040	gi\|120497372	glutaredoxin-1	P. pachyrhizi	9	323
PHAP0048	gi\|120503688	heat shock HSS1	P. pachyrhizi	7	376
PHAP0069	gi\|120509299	NADH-cytochrome b5	P. pachyrhizi	5	321
		reductase
PHAP0085	gi\|120514870	polyubiquitin	P. pachyrhizi	12	410
38. Transposable elements
PHAP0105	gi\|254151689	transposon	M. larici-populina	1	47
99 Unclassified
PHAP0030	gi\|120518530	cytoplasm protein	P. pachyrhizi	5	313
PHAP0129^2,3	gi\|120521555	hypothetical protein	P. pachyrhizi	3	111
PHAP0051	gi\|120523258	hypothetical protein	P. pachyrhizi	2	191
PHAP0052³	gi\|120510614	hypothetical protein	P. pachyrhizi	12	289
PHAP0093	gi\|120519941	hypothetical protein	P. pachyrhizi	15	512
PHAP0053	gi\|120534989	hypothetical protein	P. pachyrhizi	5	554
PHAP0054²	gi\|103096425	hypothetical protein	U. maydis	3	65
PHAP0055	gi\|120521631	hypothetical protein	P. pachyrhizi	5	172
PHAP0057	gi\|120524713	hypothetical protein	P. pachyrhizi	5	141
PHAP0058	gi\|120506400	hypothetical protein	P. pachyrhizi	10	190
PHAP0059³	gi\|120508009	hypothetical protein	P. pachyrhizi	3	119
PHAP0060	gi\|120518154	hypothetical protein	P. pachyrhizi	15	402
PHAP0061	gi\|120519874	hypothetical protein	P. pachyrhizi	4	98
PHAP0062	gi\|120519876	hypothetical protein	P. pachyrhizi	6	194

¹Predicted Pha kopsora p achyrhizin protei (PHAP).
²Proteins identified from the appressoria water fraction.
³Proteins containing a secretion signal.

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ISSN: 1471-2164

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