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Table 4 Estimating transcriptome completeness by comparison to Nematostella a

From: Developing the anemone Aiptasia as a tractable model for cnidarian-dinoflagellate symbiosis: the transcriptome of aposymbiotic A. pallida

Pathway Number of predicted proteins assigned to this pathway in Nematostella Number of orthologs in Aiptasia transcriptome Average% of Nematostella coding sequence covered by best alignment Average predicted amino acid similarity (%)b
Glycolysis and gluconeogenesis 30 25 89 87
Amino-sugar and nucleotide-sugar metabolism 28 25 68 85
Regulation of autophagy 13 10 63 85
Pentose-phosphate pathway 18 14 81 89
Citrate cycle 28 24 90 89
Valine, leucine, and isoleucine degradation 37 34 80 88
Purine metabolism 92 78 62 86
Fatty acid biosynthesis 5 3 62 79
  1. aAiptasia orthologs to Nematostella proteins in each pathway (as identified in KEGG) were predicted by best-reciprocal-BLAST analysis (see text).
  2. b Using BLOSUM62 similarity matrix [41].