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Table 5 Completeness of transcripts and sequence conservation for some proteins involved in cellular spatial organization

From: Developing the anemone Aiptasia as a tractable model for cnidarian-dinoflagellate symbiosis: the transcriptome of aposymbiotic A. pallida

Query protein (GenBank Accession Number) Amino acids in query sequence Amino acids of query covered by best BLAST hit(s) % amino-acid sequence identity (number of gaps) Length of Aiptasia contig (bp) Positions in contig covered by best BLAST hit
Mouse Cdc42 (P60766) 191 1-191 92 (0) 1,399 275-847
Mouse cyto-plasmic actin 1 (P60710) 375 2-375 97 (0) 1,455 117-1,247
Mouse tubulin α1B (P05213) 451 1-432 97 (0) 1,971 1,879-584
Mouse tubulin β5 (P99024) 444 1-427 98 (0) 2,592 1,529-249
Mouse septin-2 (P42208) a 361 139-329 65 (14b) 1,979 3-617
Mouse kinesin 1 heavy chain (Q61768) 963 3-199 72 (0) 906 592-2
   453-947 53 (14) 2,138 6–1,514
Mouse myosin 8 (P13542) 1,937 23-1,903 50 (14) 7,798 7,650-2,032
Mouse dynein heavy chain 1 (Q9JHU4) 4,644 21-216 53 (4) 790 191-790
   836-4,643 73 (30) 11,600 3–11,414
  1. a Our unpublished studies have shown that as expected, the A. pallida genome encodes multiple septins and that, like other septins [42], these contain the three conserved motifs of a GTP-binding site in their N-terminal regions. We do not yet know why none of these transcripts appears in full-length form in the current transcriptome.
  2. b The predicted A. pallida protein has a single insertion of 14 amino acids near the C-terminus of the “septin-unique element” [42].