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Table 5 Completeness of transcripts and sequence conservation for some proteins involved in cellular spatial organization

From: Developing the anemone Aiptasia as a tractable model for cnidarian-dinoflagellate symbiosis: the transcriptome of aposymbiotic A. pallida

Query protein (GenBank Accession Number)

Amino acids in query sequence

Amino acids of query covered by best BLAST hit(s)

% amino-acid sequence identity (number of gaps)

Length of Aiptasia contig (bp)

Positions in contig covered by best BLAST hit

Mouse Cdc42 (P60766)

191

1-191

92 (0)

1,399

275-847

Mouse cyto-plasmic actin 1 (P60710)

375

2-375

97 (0)

1,455

117-1,247

Mouse tubulin α1B (P05213)

451

1-432

97 (0)

1,971

1,879-584

Mouse tubulin β5 (P99024)

444

1-427

98 (0)

2,592

1,529-249

Mouse septin-2 (P42208) a

361

139-329

65 (14b)

1,979

3-617

Mouse kinesin 1 heavy chain (Q61768)

963

3-199

72 (0)

906

592-2

  

453-947

53 (14)

2,138

6–1,514

Mouse myosin 8 (P13542)

1,937

23-1,903

50 (14)

7,798

7,650-2,032

Mouse dynein heavy chain 1 (Q9JHU4)

4,644

21-216

53 (4)

790

191-790

  

836-4,643

73 (30)

11,600

3–11,414

  1. a Our unpublished studies have shown that as expected, the A. pallida genome encodes multiple septins and that, like other septins [42], these contain the three conserved motifs of a GTP-binding site in their N-terminal regions. We do not yet know why none of these transcripts appears in full-length form in the current transcriptome.
  2. b The predicted A. pallida protein has a single insertion of 14 amino acids near the C-terminus of the “septin-unique element” [42].