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Figure 3 | BMC Genomics

Figure 3

From: Visualizing spatiotemporal dynamics of apoptosis after G1 arrest by human T cell leukemia virus type 1 Tax and insights into gene expression changes using microarray-based gene expression analysis

Figure 3

Time-lapse imaging of morphological changes in HeLa/Fucci2 cells after Tax-induced cell cycle arrest at G 1 phase. (A) HeLa cells were transfected with the pCAGGS-Tax-IRES-CFP vector or the control pCAGGS-IRES-CFP vector. At 24 h after transfection, cells were stained with an anti-Flag MAb followed by an Alexa Fluor 594-conjugated secondary MAb and analyzed by confocal laser scanning microscopy (Olympus FV1000). Cells showing red and blue fluorescence express Tax-Flag and CFP, respectively. (B and C) HeLa/Fucci2 cells were transfected with the CAGGS-Tax-IRES-CFP vector or the control pCAGGS-IRES-CFP vector and monitored by time-lapse photography using the Olympus LCV110 Imaging System. One day after transfection, CFP-positive cells were selected and fluorescence and phase images were captured once every 15 min for 2 days. Cells showing orange or green fluorescence are in the G1 or S/G2/M phase of cell cycle, respectively. Apoptotic cells, which show a rounded morphology, are marked by arrows. The populations of CFP-expressing cells at the G1 and S/G2/M phases (D and E, respectively) were quantified using MetaMorph 7.7.4 software

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