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Table 6 T-cell stimulating capacity of DQ2-γ-I is influenced by deamidation pattern and flanking amino acids

From: Celiac disease T-cell epitopes from gamma-gliadins: immunoreactivity depends on the genome of origin, transcript frequency, and flanking protein variation

1 2 3 4 5 6 7 8 9 SI Peptide no.
P Q Q S F P Q Q Q + 1,4
P Q Q P F P Q Q Q + 3
P Q Q S F P Q Q Q ++ 2
P Q Q S F P Q Q Q - 5,12,13
P Q Q S S P Q Q Q - 6,7
S Q Q S S P Q Q Q - 8
  1. Stimulation of a T-cell clone (SIM 114, DQ2.5) specific for CD epitope DQ2-γ-I, with deamidated 17 mer peptides (see Table 5) that are representing natural occurring variants of DQ2-γ-I found in γ-gliadin transcripts of bread wheat (ABD, T. aestivum) and ancestrally related diploid wheat genomes (Ab, S and D). Peptide no. corresponds to the peptide numbering in Table 5. Glutamine residues that are a primary targets for the enzyme tissue transglutaminase are underlined (Q) in QxP target sites whereas moderate target sites are depicted in italics ( Q ) [13, 14]. The stimulation is mediated by HLA-DQ2 carrying antigen presenting cells (APC). SI = Stimulation index = counts per minute (cpm) of the stimulated culture/cpm unstimulated culture (with APC only); ++ = SI ≥ 50, + = 20 ≤ SI <50, ± =10 ≤ SI <20, - = SI <10.