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Table 5 Potential gga-miR-146a targets

From: Integrated analysis of microRNA expression and mRNA transcriptome in lungs of avian influenza virus infected broilers

Symbol/GI

gga-miR-146a:mRNA(3’UTR) interaction

miRanda score/energy (kcal/mol)

Binding site

Insert location*

HIF1AN/118092762

3' UUGGGUACCUUAAGUCAAGAGU 5'|:|:: ||| || ||||||||5'AGCTTCTGG--TTGAGTTCTCA 3'

167/-18.0

2261-2280

1959-2417

PDHB/118097022

3' UUGGG--UACCUUAAGUCAAGAGU 5'|:||: | || | ||||||||||5'AGCCTAAAAGGCA-TCAGTTCTCA 3'

168/-22.3

3732-3754

3365-3864

LATS1/118088356

3' UUG--GGUACCUUAA----GUCAAGAGU 5'|:| |::||||| | :||||||||5' AGCTGCTGTGGAAATGGCATAGTTCTCA 3'

167/-20.5

4243- 4270

4067-4381

POU1F1/45383513

3' UUGGGUACCUUAAGUCAAGAGU 5'| :|: | |: | ||||||||5'ACTCTCTTCAGGT-AGTTCTCA 3'

150/-16.5

2979-2999

2327-3057

CHMP2B/71896762

3' UUGGGUAC-CUUAAGUCAAGAGU 5'|| :| || |||| |||||||||5'AAGTC-TGAGAATGCAGTTCTCA 3'

174/-21.1

1893-1914

1778-2027

ARL11/118084874

3' UUGG-GUA---CCUUAAGUCAAGAGU 5'||| ||| ||| ||||||||5'GACCGCATATAGGA----AGTTCTCA 3'

157/-19.1

1579-1600

1424-1694

MAP3K3/118102843

3' UUGGGU----A-CCUUAAG----UCA-AGAGU 5'::|||| | ||||| : ||| |||||5'GGCCCAAGAGTGGGAATGTAAGAAGTGTCTCA 3'

127/-19.6

2235-2266

2057-2320

  1. Note: * The 3’UTR predicted target genes containing gga-miR-146a binding sites were cloned into the 3’UTR of the psiCHECK-2 vector (Promega).
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BMC Genomics

ISSN: 1471-2164

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