Table 1 Restriction-site associated DNA (RAD) sequencing statistics summary of a Kpn I library from eight B. napus inbred lines
Statistic | Number |
|---|---|
Total Illumina reads, x 106 | 26.47 |
Total reads containing barcode | Â |
x 106 (1 mismatch allowed) | 26.33 |
Total reads containing restriction | Â |
overhang x 106 (1 mismatch allowed) | 25.16 |
Reads per inbred containing restriction | Â |
overhang x 106 (1 mismatch allowed) (range) | 2.08-4.14 |
RAD tags per inbred with <200 | Â |
reads (range) | 100,420-127,681 |
Mean of reads per RAD tag with ≤200 reads (range) | 13.74-22.43 |
Median of reads per RAD tag with ≤200 |  |
reads (range) | 10-17 |
Total RAD clusters | 113,221 |
Total polymorphisms | 32,839 |
Single nucleotide polymorphisms (SNPs) | Â |
from positions 7-88 bp | 20,835 |
RAD clusters from which SNPs were derived | 9,552 |
Insertions/Deletions (InDels) from positions 7-80 bp | 125 |
RAD clusters from which InDels were derived | 59 |
Reads per SNP-inbred combination (median) | 9.7 |
Reads per InDel-inbred combination (median) | 1.0 |