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Table 1 Restriction-site associated DNA (RAD) sequencing statistics summary of a Kpn I library from eight B. napus inbred lines

From: High-throughput polymorphism detection and genotyping in Brassica napus using next-generation RAD sequencing

Statistic Number
Total Illumina reads, x 106 26.47
Total reads containing barcode  
x 106 (1 mismatch allowed) 26.33
Total reads containing restriction  
overhang x 106 (1 mismatch allowed) 25.16
Reads per inbred containing restriction  
overhang x 106 (1 mismatch allowed) (range) 2.08-4.14
RAD tags per inbred with <200  
reads (range) 100,420-127,681
Mean of reads per RAD tag with ≤200 reads (range) 13.74-22.43
Median of reads per RAD tag with ≤200  
reads (range) 10-17
Total RAD clusters 113,221
Total polymorphisms 32,839
Single nucleotide polymorphisms (SNPs)  
from positions 7-88 bp 20,835
RAD clusters from which SNPs were derived 9,552
Insertions/Deletions (InDels) from positions 7-80 bp 125
RAD clusters from which InDels were derived 59
Reads per SNP-inbred combination (median) 9.7
Reads per InDel-inbred combination (median) 1.0