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Figure 2 | BMC Genomics

Figure 2

From: Transcriptome characterization via 454 pyrosequencing of the annelid Pristina leidyi, an emerging model for studying the evolution of regeneration

Figure 2

Effectiveness of normalization of the cDNA library using duplex-specific nuclease. (A) Agarose gel smears show that non-normalized cDNA (treated with water) has distinct bands representing highly expressed genes, but these bands are absent in the normalized sample treated with duplex-specific nuclease (DSN). (B) RT-PCR analysis of transcript levels indicates that representation of the highly expressed genes Pl-β-actin and Pl-α-tubulin in the library is decreased after normalization with DSN. Representation of three lowly expressed genes, Pl-wnt-1, Pl-otx-2, and Pl-hox-Z, is increased, consistent with successful normalization. Standard error bars are shown.

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