Figure 5

Validation of transcript assembly for two isogroups. Contigs and isotigs produced by the assembly are shown for two isogroups, (A) a putative piwi-like gene and (B) a putative frizzled gene. Blue boxes represent major contigs and V-shaped lines connect contigs that are adjacent to each other within the isotig. Short contigs of only a few nucleotides are omitted in this representation. Contigs that were independently confirmed by PCR and sequencing are represented as filled blue boxes (as opposed to open boxes) and connections between contigs that were independently confirmed by PCR and sequencing are indicated by solid V-shaped lines (as opposed to dotted lines). All major contigs and one isotig for each gene (isotigs 33900 (A) and 19226 (B)) were validated. Nucleotide sequence alignments are provided in Additional file 4.