Table 2 Summary of experimental procedure for sRNA libraries construction
From: Computational identification and analysis of novel sugarcane microRNAs
Assay | Culture condition | Genotype | Sample | sRNA libraries |
|---|---|---|---|---|
Water deficita | soil | Tolerant - CTC15, CTC6, SP83-2847 and SP83-5073 | root | Control: T0h and S0h |
Sensitive - CTC9, CTC13, SP90-1638 and SP90-3414 | T24h and S24h | |||
Salt stressb | in vitro - hydroponic | SP70-1143 | leaf | Control (0 h) |
1 h; 6 h; 24 h | ||||
Pathogen infectionc | in vitro | SP70-1143 | whole plant | Control (CT) |
Inoculated (AA) |