Validation of RNA-seq results by real-time PCR. Transcript abundance represented by the two heat maps are the average of transcript abundance values from three independent real-time PCR experiments (left) and RPKM values of the RNA-seq analysis (middle). For each gene, the tissue with the maximum transcript abundance was set to 100, and relative transcript abundances in the other three tissues were calculated based on this maximum. Relative expression is represented by color scales as indicated (right). R is the correlation coefficient value between the two platforms.