Figure 3

Gene expression profiling of BMP2 and TSA treated neurosphere cultures. Differentially expressed genes (relative expression > 2-fold and absolute difference >100, comparing treated and control sample, confidence > 90%) are presented as Venn-diagrams (B-G) and hierarchical cluster (H). Venn diagrams present the number of genes regulated upon TSA and BMP2 treatment after 6 h and 24 h. Numbers of regulated genes are indicated in the diagram, the total number of regulated genes for each treatment is put in parentheses. Intersection of regulated genes between TSA 6 h and BMP2 6 h (B), TSA 24 h and BMP2 6 h (C), TSA 6 h and BMP2 24 h (D), TSA 24 h and BMP2 24 h (E), BMP2 6 h and BMP2 24 h (F) and TSA 6 h and TSA 24 h (G) are illustrated. Differentially expressed genes are clustered in a hierarchical cluster analysis. Red and green depict at least two-fold increase or decrease in expression, respectively. Clusters including upregulated genes are labeled at the right side of the cluster plot. Clusters, grouping genes downregulated after treatment, are labeled on the left side (H). Genes from individual main sub-cluster of hierarchical cluster analysis were functional annotated using DAVID Database.