De novo transcriptome sequencing in a songbird, the dark-eyed junco (Junco hyemalis): genomic tools for an ecological model system

Table 3 Additional annotation approach details

Approach	Database	Blast Flavor	E-value	References	Notes
Functional Annotation	NCBI- NR	Blast2GO	10^-15	[41, 42, 47, 51]
Pathway Annotation	KEGG	tBlastX	10^-5	[52–54]	Limited to zebra finch, chicken, and mouse.
Orthologous Group	OrthoDB, Ensembl	BlastX	10^-10	[35–37, 56]	Limited to zebra finch. Matched Ensembl hits to OrthoDB assignments

To obtain more detailed information about the function of the sequenced genes, searches beyond simple non-redundant proteins were conducted.

ISSN: 1471-2164