Figure 3From: Let-7b regulates the expression of the growth hormone receptor gene in deletion-type dwarf chickensDual-luciferase reporter assay for validation of the 3′ UTR of GHR as the target site of the let-7b in vitro cell system. DF-1 cells were transfected with pmirGLO-let-7b-GHR 3′ UTR, pmirGLO-let-7b-GHR 3′ UTR mutation plasmid, or the control plasmid. The activity of firefly luciferase (luc2) and Renilla luciferase (hRluc-neo) was measured and relative values (luc2/hRluc-neo) calculated.Back to article page