Quantitative RT-PCR showing sexual dimorphic expression at stage 17 and 19. The expression of MALAT1 (n = 5) consists of sex and stage (e.g. M17 = male, stage 17) and is relative to the control gene (PP1). The relative expression for M17 was set as 1 to allow easier comparison between groups. Gene expression was analysed using two-tailed t-test to examine differences between sexes at the same stage (stage 17, P = 0.04 and stage 19, P = 0.01) and between stages of the same sex (male, P = 0.18 and female, P = 0.24).