Table 3 Semi-quantitative and quantitative real-time PCR primers, and optimal conditions for semi-quantitative PCR
Gene symbol | Primer name | Primer Sequence | Annealing Temperature (°C) | MgCl2Concentration (mM)* | Cycle Number |
|---|---|---|---|---|---|
Cct3 | Cct3 F | GGATGCCTAAAATTAGCCTCCTA | 62.5 | 1.5 | 30 |
Cct3 R | GAAGCTACGGCAAATGATGG | ||||
Malat1§ | Malat1 F | GTACGCGGGCAGACTAACAC | 57.1 | 1.5 | 36 |
Malat1 R | TGCGTCTAGACACCACAACC | ||||
C16orf62 | C16orf62 F | CGGCCGAGGTACAAATTAAG | 58.3 | 2.5 | 36 |
C16orf62 R | TGCAAGTGCATTATGGAAGC | ||||
Mmp2 | Mmp2 F | ATGAAGAAGCCCCGCTGTGGTAATCC | 62.5 | 4.5 | 27 |
Mmp2 R | AAAGGCATCGTCTACTGTTTCGGAGTCC | ||||
Nf1a | Nfib F | AAACACACTGCGTCAAGTGC | 61.4 | 1.5 | 24 |
Nfib R | CTTGCCCTGGATAGCGATTA | ||||
Notch2 | Notch2 F | TATTTCTGTGGCTGCCTGGA | 62.5 | 1.5 | 36 |
Notch2 R | GGGACAGGGACCTTTGTTGT | ||||
Pp1§ | Pp1 F | ACCTCTTCCTGGGCGACTAT | 62.5 | 1.5 | 27 |
Pp1 R | TGATGTTGTAGCGCCTCTTG |