Figure 4
An analytical view of the phylogenetic relationship among the rice OsGELP and plant homologues of known function. Protein NJ tree: The unrooted tree, constructed using ClustalW, summarizes the evolutionary relationship among 120 members of the GDSL esterase/lipase plant family. The NJ tree was constructed using the alignment of only the highly conserved amino acid sequence regions. The tree shows 13 major phylogenetic groups. Left column identifies subclades and is marked with different alternating tones of background to make subclade identification easier. The numbers beside the branches represent bootstrap values based on 1,000 replications. The node numbers lower than 50 are not shown. Protein motif structure and location: the OsGELP and plant GDSL esterase/lipase proteins are in the order of their appearance in the phylogenetic tree. Each coloured box represents particular motif. Their consensus sequence, length (amino acids), number of the GDSL esterase/lipase proteins containing the motif, and E-value are given in Additional file 11. The GDSL motif blocks I, II, III, and V are indicated in pink boxes above the motif distribution pattern. The length of proteins (amino acids) can be estimated using the scale at the bottom. Motifs enclosed in red, blue, or green frames are highlighted motifs that exclusively appear in proteins from one, two, or three subclades, respectively. The number of highlighted motifs specific for one or several subclades is given at the right. The secondary element assignment, below the motif distribution scheme, corresponds to the general structure of the OsGELPs.