Figure 2

Validation of Auxin Response Factors (ARFs) regulated by gma-miR160 in cotyledon. Confirmed targets (a-d) for gma-miR160 are presented in the form of target plots (t-plot) and alignments. Absolute numbers of signature sequences are indicated in the t-plot. Red arrows indicate signatures consistent with miRNA-directed cleavage. The black arrows indicate a site verified by RLM 5’-RACE and detected cleavage frequencies (absolute numbers) are shown above the arrow. The cleavage site is shown as a red letter. Cleavage frequency as determined by gene-specific 5’-RACE at the indicated position is shown in parenthesis.