Identification of soybean seed developmental stage-specific and tissue-specific miRNA targets by degradome sequencing

Table 1 Analysis of degradome reads from five different libraries matched to the soybean genome

Library name^a	Raw Reads	20-21 nt reads	Genome matched reads^b	Transcriptome matched Reads^b
Cot25	27802207	25926963	25300772	24754628
SC25	41395866	40296710	39308067	37957708
Cot100	10473739	9088079	8902528	8646558
SC100	26326261	25931744	25289389	24436953
Cot300	44451740	41517379	40494429	40335132

^aCot25 and SC25 are libraries from cotyledons and seed coats dissected from early-maturation green seed of 25–50 mg fresh wt.; Cot100 and SC100 are cotyledons and seed coats dissected from mid-maturation green seed of 100–200 mg fresh weight range; Cot300 are cotyledons dissected from late maturation yellow seeds of 300–400 mg weight range.
^bGenome matched reads indicate the number of reads of 20 or 21 nt that matched to Glycine max genome sequence available in Phytozome. Transcriptome matched reads indicate the number of reads that matched to Glycine max transcriptome (Glyma models) available in Phytozome [23].

ISSN: 1471-2164