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Table 2 Flux distributions as a function of NADPH demand

From: A comparative transcriptomic, fluxomic and metabolomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation

Acetoin (mM)

0

100

200

300

Glucose uptake (mmol/gDW/h)

17.8

19.5

19.6

25.7

Glycolysis

84.7 ± 0.9

78.8 ± 1.9

69.9 ± 0.6

65.2 ± 0.6

PPP

11.4 ± 0.9

18 ± 1.9

26 ± 0.9

33.0 ± 1.4

Transketolase

5.6 ± 1.5

9.0 ± 2.5

12.2 ± 2.7

18.6 ± 4.2

Biomass (Carbohydrates)

3.3 ± 0.1

3.4 ± 0.1

2.5 ± 0.1

2.1 ± 0.1

Pyruvate decarboxylase

164.3 ± 0.1

164.8 ± 0.5

167.1 ± 0.1

167.9 ± 0.4

Pyruvate carboxylase

4.2 ± 0.8

2.7 ± 0.7

2.1 ± 0.6

2.5 ± 0.6

Import of oxaloacetate into the mitochondria

3.4 ± 0.8

2 ± 0.7

1.6 ± 0.6

2 ± 0.6

Acetaldehyde dehydrogenase

2.4 ± 0.1

3.8 ± 0.06

4.2 ± 0.1

5.6 ± 0.1

Biomass (Lipids)

0.29 ± 0.01

0.17 ± 0.02

0.15 ± 0.02

0.09 ± 0.04

Acetyl-CoA synthetase

0.30 ± 0.03

0.20 ± 0.06

0.22 ± 0.1

0.13 ± 0.09

Ethanol production

161.9 ± 0.1

161 ± 0.5

162.9 ± 0.5

162.3 ± 0.5

Glycerol production

17.8 ± 0.1

17.9 ± 0.1

14.7 ± 0.1

13.7 ± 0.1

Acetate production

2.1 ± 0.1

3.6 ± 0. 1

4.0 ± 0. 1

5.5 ± 0.1

Succinate production

0.30 ± 0.01

0.26 ± 0. 01

0.26 ± 0. 01

0.28 ± 0. 01