Figure 2

Testing of sequence-specific PCR-based molecular markers “AnSeq1”, “AnSeq2” and “AnSeq5” on 26 F ₈ recombinant inbred lines from a cross of Unicrop (susceptible to anthracnose disease) x Tanjil (resistant) of lupin ( Lupinus angustifolius L .). “AnSeq1^R”, “AnSeq2^R” and“AnSeq5^R” indicate the marker allele bands linked to disease resistance gene Lanr1. “AnSeq1^S”, “AnSeq2^S” and “AnSeq5^S” indicate the marker allele bands associated with disease susceptibility allele. Disease phenotypes of the RILs are presented as “S” (susceptible) or “R” (resistant). A marker band with a vertical arrow indicates that a genetic recombination occurred between the R gene and marker locus on the chromosome in that particular plant for that particular marker. All other un-marked marker bands showed the correct match between the marker genotypes and the disease resistance phenotypes on these testing plants.