Figure 6

qPCR analysis of the genes related to MHC-II processing pathway in zebrafish following WED - immunization during the first five days. Lysosomal membrane glycoprotein 2 (lamp2), MHC class II DAB gene (MHC-II dab), CD74 molecule (cd74) and MHC class II, transactivator (CIITA) were validated. qPCR analysis for the expressions of the above immune-related genes in livers (gray bars) and spleens (white bars) of zebrafish vaccinated with WED. The experimental procedure was the same as in the legend of Figure 5.