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Table 3 43 Variable genes found within a clonal lineage of Salmonella Montevideo.

From: High resolution clustering of Salmonella enterica serovar Montevideo strains using a next-generation sequencing approach

Gene

LT Locus

Drain Locus

Nuc

AA

Position

Clade

Feature

lig

STM2427

SEEM020_00085

C/T

T

1974

b

DNA ligase, NAD-dependent

perM

STM2493

SEEM020_00410

C/T

A

1034

d,e,f

permease PerM

aroB

STM3486

SEEM020_01090

C/T

V/A

488

b,c,d,e,f

shikimate kinase I

yrfI

STM3498

SEEM020_01145

C/T

T/I

353

b,c,d,e,f

heat shock protein

gntK

STM3542

SEEM020_01330

G/A

H/Q

93

a1

1) gluconate transporter GntU, 2) shikimate kinase

dppA

STM3630

SEEM020_01925

C/T

H

282

a1

dipeptide transport protein

tolB

STM0748

SEEM020_01960

G/T

A/S

64

a1

tolB protein precursor

citG

STM0619

SEEM020_04239

C/T

R/C

181

b,c,d,e,f

triphosphoribosyl-dephospho-CoA synthase

citF

STM0621

SEEM020_04249

C/T

V/A

602

b,c,d,e,f

citrate lyase alpha chain

ydfZ

STM1509

SEEM020_04749

G/A

P

174

b

putative selenium-binding protein YdfZ

 

STM1546

SEEM020_04939

C/T

L

1473

d,e,f

1) putative multidrug efflux protein, 2) hypothetical protein

 

SeSA_A1664

SEEM020_05139

C/T

L

667

a1

LysR substrate binding domain protein

 

STM1627

SEEM020_05529

C/T

T

543

a1

alcohol dehydrogenase class III

 

STM1628

SEEM020_05534

T/G

L/R

155

a1

putative cytoplasmic protein

 

STM1671

SEEM020_05759

A/C

V

122

a1

putative regulatory protein

 

STM1856

SEEM020_06993

G/T

E/Stop

316

 

putative cytoplasmic protein

fliC

STM1959

SEEM020_07518

T/A

N/K

723

a1

phase 1 flagellin

uhpA

STM3790

SEEM020_08264

A/G

l

60

a1

1) sensor histidine kinase UhpB, 2)transcriptional regulatory protein UhpA

nuoL

STM2318

SEEM020_09061

C/T

F

291

f2

NADH dehydrogenase I

 

STM4534

SEEM020_10120

C/T

A/V

14

b

putative transcriptional regulator

ytfG

STM4401

SEEM020_10825

C/T

S/F

503

f3

conserved hypothetical protein

yjeM

STM4345

SEEM020_11085

C/T

L

1179

a1

putative APC family amino-acid transport protein

 

STM4261

SEEM020_11575

G/A

V/I

4684

 

putative inner membrane protein

araD

STM0101

SEEM020_12590

C/A

Q/K

466

b

L-ribulose-5-phosphate 4-epimerase

araB

STM0103

SEEM020_12600

G/A

L

702

e,f

L-ribulokinase

 

STM3260

SEEM020_13557

G/A

V/M

70

b

PTS family galactitol-specific enzyme IIC

 

SeD_A3648

SEEM020_13697

A/G

D/G

263

d,e,f

hypothetical protein

pduV

STM2056

SEEM020_14356

G/A

D/G

353

b,c,d,e,f

propanediol utilization protein

orf408

STM1382

SEEM020_15066

G/A

T/A

1096

a1

putative regulatory protein, deoR family

ydiA

STM1348

SEEM020_15231

C/T

F

126

b,c,d,e,f

putative inner membrane protein

envE

STM1242

SEEM020_15746

T/C

I/T

446

a1

EnvE

ycfX

STM1220

SEEM020_15941

C/T

G

24

d,e,f

N-acetylglucosamine kinase

 

STM4097

SEEM020_16375

G/A

S/N

119

a1

putative outer membrane lipoprotein

uvrD

STM3951

SEEM020_17067

G/A

G/E

2111

f2

DNA helicase II

 

STM2404

SEEM020_17529

G/T

A/S

394

b

putative chloride channel permease

recB

STM2994

SEEM020_17950

G/A

S/G

901

d,e,f

exodeoxyribonuclease V

stdB

STM3028

SEEM020_18130

C/T

L

2433

f2

putative outer membrane usher protein

yqjI

STM3215

SEEM020_19095

C/A

H/N

562

e,f

1) transcriptional regulator (PadR), 2) family methyl-accepting chemotaxis protein II

ybhK

STM0801

SEEM020_19330

G/A

L

618

a1

conserved hypothetical protein

 

STM0818

SEEM020_19410

G/T

A/S

277

e,f

1) putative ABC-type multidrug transport system, 2) membrane permease predicted cation efflux pump

yliD

STM0851

SEEM020_19565

T/C

W/R

760

 

putative ABC transporter inner membrane component

invE

STM2897

SEEM020_21151

C/G

L/V

757

f

invasion protein

yejM

STM2228

SEEM020_21392

G/A

A/E

395

a1

putative hydrolase of alkaline phosphatase superfamily

  1. Variable genes are listed by their Genbank abbreviated and full name (feature) and by the blast locus hit to either a reference isolate LT2 or the Drain swab isolate. A representative nucleotide change observed within each gene is listed as well as whether this caused an AA change and to which phylogenetic group it was associated with from Figure 3 (A-F). These SNPs were the most useful for the spiced meat outbreak investigation and will be useful for both targeted resequencing efforts and for rapid subtyping methods for traceback of future S. Montevideo investigation and diagnosis.