Skip to main content

Table 1 Physical maintenance conditions for the three Dehalococcoides -containing enrichment cultures

From: Comparative metagenomics of three Dehalococcoides-containing enrichment cultures: the role of the non-dechlorinating community

  KB-1 DonnaII ANAS
Cultures    
Liquid volume 1.6 L 5.7 L 0.4 L
Stirred No Yes Yes
Temperature 20-22 °C 30 °C 25-28 °C
Electron acceptor TCE (858 μmol added/L)1 PCE (110 μmol added/L)2 TCE (278 μmol added/L)3
Electron donor Methanol (4.3 mM)1 Butyrate (440 μM)2 Lactate (25 mM)3
Feeding frequency 14 days 2 days 4-7 days
Residence time 2 years4 70-80 days5 16-47 days3
Donor loading rate [(μmol/L/d)/(meeq/L/d)] 0.31/1.84 0.22/4.44 4.55/54.5
Acceptor loading rate [(mmol/L/d)/(meeq/L/d)] 0.061/0.37 0.055/0.44 0.051/0.30
Donor eeq/Acceptor eeq 5 10 180
Cobalamin amended B12 (0.005 μg/L) B12 (1 μg/L) B12 (0.1 μg/L)
Dhc strain(s) KB-1/PCE & KB-1/VC1 DET1955 ANAS (2 strains) 3
  1. For electron equivalent ratios (eeq), the following eeq were utilized: PCE = 8, TCE = 6, butyrate = 18, lactate = 6, methanol = 8.
  2. 1 -[1]
  3. 2 -[29]
  4. 3 -[28]
  5. 4 - in-house data, medium exchange: 25% of volume roughly each 6 months.
  6. 5 -[20]
\