Table 1 Physical maintenance conditions for the three Dehalococcoides -containing enrichment cultures
| Â | KB-1 | DonnaII | ANAS |
|---|---|---|---|
Cultures | Â | Â | Â |
Liquid volume | 1.6 L | 5.7 L | 0.4 L |
Stirred | No | Yes | Yes |
Temperature | 20-22 °C | 30 °C | 25-28 °C |
Electron acceptor | TCE (858 μmol added/L)1 | PCE (110 μmol added/L)2 | TCE (278 μmol added/L)3 |
Electron donor | Methanol (4.3 mM)1 | Butyrate (440 μM)2 | Lactate (25 mM)3 |
Feeding frequency | 14 days | 2 days | 4-7 days |
Residence time | 2 years4 | 70-80 days5 | 16-47 days3 |
Donor loading rate [(μmol/L/d)/(meeq/L/d)] | 0.31/1.84 | 0.22/4.44 | 4.55/54.5 |
Acceptor loading rate [(mmol/L/d)/(meeq/L/d)] | 0.061/0.37 | 0.055/0.44 | 0.051/0.30 |
Donor eeq/Acceptor eeq | 5 | 10 | 180 |
Cobalamin amended | B12 (0.005 μg/L) | B12 (1 μg/L) | B12 (0.1 μg/L) |
Dhc strain(s) | KB-1/PCE & KB-1/VC1 | DET1955 | ANAS (2 strains) 3 |