Differential gene expression profiling of porcine epithelial cells infected with three enterotoxigenic Escherichia coli strains

Table 2 Validation of microarray results by real-time quantitative PCR

Gene expression fold change after ETEC infection in each group
(Inf./Non.)
C_F4abvs control^*				C_F4acvs control				C_F18acvs control
Genes	Probe Name in Micro-array	Micro-array	Q-PCR^#	Genes	Probe Name in Micro-array	Micro-array	Q-PCR	Genes	Probe Name in Micro-array	Micro-array	Q-PCR
IL8	A_72_P232367	5.03**	4.09*(G) 5.50(A)	MUC20	A_72_P440416	−1.33*	−1.82**(G) −1.23(A)	FUT1	A_72_P146581	1.13	1.00(G) 1.04(A)
SDC2	A_72_P098981	−2.57**	−2.84**(G) −2.06(A)	ANK3	A_72_P628816	−2.90**	−4.40**(G) −2.99(A)	CXCL2	A_72_P146411	2.31*	2.31(G) 2.29(A)
UPK2	A_72_P441773	4.27**	3.69*(G) 5.21(A)	IRG6	A_72_P443124	4.86**	4.67(G) 6.75*(A)	IRG6	A_72_P443124	2.12*	1.62(G) 1.64*(A)
MUC4	A_72_P140196	−1.46	−2.04(G) −1.28(A)	LOC10015251	A_72_P527727	−1.25	−1.91(G) −1.42(A)
				MUC13	A_72_P442057	−3.01**	−5.66(G) −3.46(A)

^* C_F4ab, IPEC-J2 cells infected with F4ab ETEC; C_F4ac, IPEC-J2 cells infected with F4ac ETEC ; C_F18ac, IPEC-J2 cells infected with F18ac ETEC ; Control: the non-infected IPEC-J2 cells. ^# G: gene expression level related to GAPDH; A: gene expression level related to ACTB. The numbers here represent the fold change of gene expression after ETEC infection. The numbers equal to 1.00 means the expression level doesn’t change after ETEC infection. The numbers > 1.00 means the expression level is increased after ETEC infection while numbers < 1.00 indicate that the expression level is reduced after ETEC infection. The numbers with “*” are statistically significant (P < 0.05), and which with“**” are statistically very significant (P < 0.01).

ISSN: 1471-2164