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Table 1 DNA extraction, PCR amplification and sequencing results

From: Microbial transformation from normal oral microbiota to acute endodontic infections

Sample Systemic or Local DNA Concentration (ng/ul) Total Volume (ul) Total DNA (ug) PCR Amplification Number of Sequencing Reads
E007 AS Local 192.40 42.00 8080.80 successful 7581
E007 OS Local 20.50 33.00 676.50 successful 10927
E007 RC Local 9.20 12.00 110.40 low PCR yield (84.21ng) 8791
E009 AS Systemic 13.60 25.00 340.00 no amplification n/a
E009 OS Systemic 33.60 32.00 1075.20 successful 11876
E009 RC Systemic 46.40 37.00 1716.80 successful 11319
E010 AS Systemic 1561.80 36.00 56224.80 low PCR yield (61.08ng) 4277
E010 OS Systemic 126.60 34.00 4304.40 successful 9435
E010 RC Systemic 21.00 35.00 735.00 successful 11504
E012 AS Local 4503.20 42.00 189134.40 low PCR yield (93.39ng) 5800
E012 OS Local 394.70 35.00 13814.50 successful 11211
E012 RC Local 67.30 40.00 2692.00 successful 11835
E016 AS Local 15.50 27.00 418.50 low PCR yield (80.46ng) 6774
E016 OS Local 19.60 27.00 529.20 successful 12651
E016 RC Local 6.50 5.00 32.50 successful 11809
E017 AS Local 199.90 40.00 7996.00 successful 10735
E017 OS Local 22.00 35.00 770.00 successful 11305
E017 RC Local 128.20 40.00 5128.00 successful 10365
E018 AS Systemic 727.20 40.00 29088.00 successful 11439
E018 OS Systemic 15.80 30.00 474.00 successful 10148
E018 RC Systemic 23.50 31.00 728.50 successful 8601
E019 AS Local 18.30 30.00 549.00 successful 16937
E019 OS Local 16.50 27.00 445.50 successful 6575
E019 RC Local 734.10 35.00 25693.50 successful 9624