Pleiotropic functions of catabolite control protein CcpA in Butanol-producing Clostridium acetobutylicum

BMC Genomics

Table 2 Correlation between microarray and qRT-PCR ^† results

Gene locus	Gene name	Function of gene product	Change fold (824ccpA/824WT)
			Middle exponential phase (M)		Stationary phase (S)
			Microarray	qRT-PCR*	Microarray	qRT-PCR*
CAC1345	xylT	l-arabinose-proton symporter	71.6	92.52 ± 2.55	147.39	326.54 ± 176.86
CAC2612	xylB	Xylulose kinase	26.9	20.4 ± 4.00	17.9	21.21 ± 0.02
CAC1348	tkt1	Transketolase	5.6	4.42 ± 0.23	7.9	6.4 ± 0.21
CAC1941	abrB1941	Stationary/sporulation gene regulator	5.1	89.17 ± 7.31	1.62	1.62 ± 0.30
CAC1660	bukII	Butyrate kinase II	4.3	7.03 ± 2.02	5.2	10.79 ± 0.04
CAC3319	-	Signal transduction histidine kinase	3.95	2.7 ± 1.01	3.43	2.41 ± 0.30
CAC2966	lacR	Lactose phosphotransferase system repressor	1.95	6.88 ± 1.43	1.13	15.49 ± 5.61
CAC3647	abrB3647	Stationary/sporulation gene regulator	0.67	0.47 ± 0.05	0.48	0.61 ± 0.11
CAC1653	-	Cell wall biosynthesis glycosyltransferase	0.43	0.34 ± 0.10	0.56	0.41 ± 0.11
CAP0164	ctfB	Butyrate-acetoacetate COA-transferase subunit B	0.27	0.17 ± 0.01	1.00^§	0.24 ± 0.06
CAC0103	cysC	Adenylylsulfate kinase	0.25	0.04 ± 0.01	182	49.14 ± 41.18
CAC0570	glcG	PTS enzyme II, ABC component	0.16	0.05 ± 0.02	0.19	0.34 ± 0.14
CAC0310	abrB310	Stationary/sporulation gene regulator	0.14	0.62 ± 0.08	0.18	0.59 ± 0.22
CAC1341	araD	l-ribulose-5-phosphate 4-epimerase	2.29	2.11 ± 0.50	5.81	2.33 ± 1.80
CAC1343	ptk	Phosphoketolase	1.12	1.84 ± 0.59	2.87	2.35 ± 0.71

^†The wild-type (824WT) and ccpA-inactivated mutant strain (824ccpA) were cultured in P2 medium using 40 g/L d-glucose and 20 g/L d-xylose as the carbon sources. Cells were harvested at middle exponential phase (A₆₀₀ ≈ 1.0) and early stationary phase (A₆₀₀ ≈ 12.0).
^* The data were mean ± standard deviation (SD) of two independent biological replicates.
^§ The microarray result of the ctfB gene at S phase was inaccurate because its expression level exceeded the detection upper limit.

ISSN: 1471-2164