Growth and phenotypic profiles of HacAWTand HacACAstrains. (A) Differences on colony size (diameter) of HacAWT and HacACA strains growing at different temperatures. 104 spores were spotted on solid CM plates and growth was monitored for 6 days. (B) Strains phenotype on CM after 3 and 6 days of growth at 30 °C. HacACA phenotype is characterized by a slower growth/colony size as well as a delay in sporulation compared to the HacAWT. Bars indicate standard deviations from three individual measurements.