Figure 4

ΔVNG0258H is impaired for growth and survival upon exposure to paraquat (PQ). (A) Mean growth rates for 7 biological replicate cultures treated in lag phase. Blue bars represent Δura3 cultures; red bars represent ΔVNG0258H cultures. (B) Representative growth curves (1 of 7 biological replicates) for cultures treated with PQ at beginning of growth phase (OD600 ≈ 0.05). Thin, medium, and thick lines indicate PQ added to a final concentration of 0, 0.167, or 0.333 mM, respectively (curve for 0.083 mM omitted for clarity). Downward arrow indicates time of PQ addition. Bracket indicates period for which mean growth rates were calculated. Line colors are as in (A). (C) Mean growth rates for 7 biological replicate cultures treated with PQ in mid-logarithmic growth phase. (D) Representative growth curves (1 of 7 biological replicates) for cultures treated with PQ in mid-logarithmic phase (OD600 ≈ 0.3). Line widths indicate the same PQ concentrations as in (B). Representative curves for 0.083 mM condition are omitted for clarity. Asterisks and error bars are as in Figure 3. All raw data are given in Additional file 3: Table S1.