Figure 2
Novel AR antagonists utilized in this study. (A) Chemical structures (compound number listed below structure). (B) Nuclear Translocation of AR was impeded by these compounds. LNAR cells were treated with 0.1nM R1881 alone or in combination with the antagonist compounds at various doses to determine IC50 values. Nuclear translocation values were calculated as indicated under Methods. (C) Treatment of VCaP cells with small molecule AR antagonist induced similar genome-wide transcriptional effects as AR inhibition by siRNA. Left: fold change from the two types of treatments; Right: SAM d-score of differential expression from the two types of treatments