Figure 3

MiRNA expression in cells and EVs. Scatter plots of the miRNA expression levels in cells and EVs of non-transfected and pSM30-miR-146a transfected cells. A) There was extremely high correlation between miRNA expression values in non-transfected and transfected cells (R² = 0.9). B) Strong correlation was also observed between EVs from non-transfected and transfected cells (R² = 0.8). C) Correlation between transfected and non-transfected cells and D) between EVs from transfected and non-transfected cells was much lower (R² = 0.6). For pSM30-miR-146a transfected cells and EVs expression levels were renormalized to RPMM following exclusion of miR-146a reads.