Figure 2
Analysis of the aqueous phase metabolites in extract of C.elegans. 1H-NMR spectrum (A) between 0 and 5 ppm and (B) between 5 and 9.5 ppm for a selected illustrative sample. Key: 1. Ile; 2. Leu; 3. Val; 4. lactate; 5. Ala; 6. Lys; 7. acetate; 8. Glu; 9. Gln; 10. Met; 11. succinate; 12. Asp; 13. Asn; 14. choline; 15. glucose; 16. Gly; 17. trehalose; 18. GTP; 19. ATP; 20. fumarate; 21. Tyr; 22. His; 23. Phe; 24. Trp; 25 formate. (C) Score plot showing the clustering pattern according to genotype in the metabolic profiles obtained by NMR spectroscopy. Results from PLS-DA following the MSCA filter on the NMR spectra of all strains. The black squares represent samples belonging to the wild type strain, the red dots samples belonging to fat-6 mutant, the blue diamonds fat-7 mutant, the green stars fat-5;fat-7 mutant, the orange triangles fat-5 mutant and the pink squares fat-5;fat-6 mutant. (D) Loading plot for the score plot in C, shows the metabolites responsible for the discrimination among the groups: results from PLS-DA following MSCA filter on the NMR spectra of all strains. (E) Score plot of PLS-DA following MSCA filter on the amino acids analysis of all strains using the Phenomenex EZFaast kit. For the key to symbols see above. (F). Loading plot of PLS-DA following MSCA filter on the amino acids analysis of all strains.