Alignment of the amino acid sequence for TSTI0006C, from T. stigmurus venom glands, with known hypotensins. Residues are numbered according to the aligned hypotensin sequences and dots represent gaps introduced to improve alignment. The underlined amino acid sequence indicates the putative signal peptide. Crosses indicate the C-terminal region probably involved in the hypotension effect. The similar BPP amino acid signature represented by a proline doublet is indicated by arrows. Pyr is the N-terminal pyroglutamic acid residue typical of snake BPPs. Green, red and gray indicate amino acids that are identical, conserved or similar, respectively. The abbreviation and GenBank accession number for the aligned hypotensin sequences are: Hypotensin-I, Tityus serrulatus (P84189), Hypotensin-II, Tityus serrulatus (P84190), BPP, Lachesis muta (ABD52884) and BPP, Bothrops jararaca (AAD51326).