Translational regulation of Anopheles gambiae mRNAs in the midgut during Plasmodium falciparum infection

BMC Genomics

Table 3 Translationally regulated immune-related genes in response to P. falciparum infection

Ensembl Gene ID	ImmunoDB Identity	Polysome loading		Transcript abundance
		Fold change (log₂)	P-value	Fold change (log₂)	P-value
AGAP005910	APG18B	2.080	0.020	−0.812	0.135
AGAP008497	APG4B	3.183	0.002	−0.881	0.199
AGAP003858	APG6	2.031	0.014	−0.269	0.614
AGAP008637	APG7A	2.953	0.000	0.169	0.853
AGAP002685	APG8	1.606	0.048	−0.425	0.249
AGAP007938	CACTUS	2.229	0.041	0.041	0.953
AGAP006473	CASPAR	4.273	0.021	−1.055	0.288
AGAP000830	CASPS7	2.563	0.012	0.764	0.487
AGAP011788	CLIPA14	4.384	0.017	−0.078	0.926
AGAP011789	CLIPA6	2.312	0.009	0.619	0.489
AGAP011792	CLIPA7	2.336	0.025	−0.392	0.717
AGAP001648	CLIPB17	3.619	0.020	0.494	0.235
AGAP004318	CLIPC3	3.574	0.024	0.842	0.331
AGAP005234	CuSOD2	2.037	0.004	−0.531	0.141
AGAP002836	DCR1	3.632	0.000	−0.462	0.465
AGAP012289	DCR2	4.277	0.001	−1.882	0.020
AGAP008087	DROSHA	5.749	0.035	0.335	0.726
AGAP004038	HPX8	3.174	0.012	0.987	0.252
AGAP007294	IAP1	3.235	0.015	−1.360	0.029
AGAP005552	PGRPLD	1.942	0.006	−1.146	0.234
AGAP006747	REL2	1.767	0.019	−0.637	0.099
AGAP012523	RM62F	2.005	0.019	−0.446	0.478
AGAP002829	SPNE	3.042	0.047	−0.562	0.504
AGAP006909	SRPN1	2.017	0.033	0.630	0.475
AGAP005246	SRPN10	1.743	0.041	−0.869	0.119

Among 1017 transcripts that are translationally regulated after ingestion of P. falciparum-infected blood, 35 transcripts have putative or established immune-related function (ImmunoDB identities). In several cases, alternate splice variants of the same gene were detected. Most of the transcripts in this list displayed significant translational change when insignificant change was observed in mRNA levels [1 ≥ (log₂mRNA_Infected- log₂mRNA_Uninfected) ≥ −1]. Some transcripts (i.e. DCR2) showed significant downregulation in transcription and upregulation in translation.

ISSN: 1471-2164