Figure 5
Resistance to  Fusarium virguliforme  and partial resistance to  Heterodera glycines  caused by the Forrest allele of a receptor like kinase (  GmRLK18-1  -a) found at the  Rfs2/Rhg1  locus as transgene in primary transgenic lines (cv ‘X5’). Panels A-H show the SDS assays. F. virguliforme was used at 104 cfu per cm3 of soil. The experiment was carried out on 3 separate occasions. Leaf scorch was scored as DS at 7, 14, 21, 28, 35, 42, 49 and 56 dai (days after infestation). Derived crossed lines (X5::GmRLK18-1-a x WENIL35 and cultivars X5:: GmRLK18-1-a x WENIL35xEF2) were included in runs 2 and 3. Panel A &B shows stable soybean transgenics with and without the 10 kbp GmRLK18-1 (Rhg1/Rfs2) subclone at 21 dai. Panel C shows the GmRLK18-1-a transgene reduced root rot at 28 dai. Panel D shows leaf symptoms at 28 dai. Panel E shows plants at 56 dai where X5 is senescent with abscission of leaflets from erect petioles and X5::GmRLK18-1-a is still green and filling pods. Panel F shows selected leaflets at 28 dai with a 1–9 range in DS scores arranged in order of severity from bottom left to top right. Panels G-J show the SCN assays. Panel G shows SCN development by the RLK in X5 transgenics. Panel H shows SCN development by the Rhg1-a allele in resistant NIL 34–23. Panel I shows normal SCN development in the susceptible X5. Panel J shows normal SCN development in susceptible NIL 34–3. Panel K shows a GmRLK18-1-a transgenic plant of cultivar X5 that was defoliated by insect herbivory in the field. Panel L shows an non-transgenic X5 plant with much less leaf area loss.