The receptor like kinase at Rhg1-a/Rfs2 caused pleiotropic resistance to sudden death syndrome and soybean cyst nematode as a transgene by altering signaling responses

Table 2 Association of mean root growth in transgenic lines with pleiotropic resistance to two pests in two different greenhouse assays and insect herbivory in field tests

A. Line::gene	SCN infested	Root mass (g)	Significant differences	Range (g)	n	SCN FI (%)
X5	No	1.05	a	0.81–1.44	15	0 ± 0.0
Westag97	No	nd		nd	5	0 ± 0.0
X5	Yes	0.98	a	0.73–1.31	15	100 ± 13
X5::RLK	No	0.64	bc	0.57–0.74	15	0 ± 0.0
X5::RLK	Yes	0.38	c	0.26–0.49	15	60 ± 11
X5::RLK::Rhg4	Yes	nd		nd	2	11 ± 3
X5::RLK::rhg4	Yes	nd		nd	3	38 ± 6
Westag97	Yes	nd		nd	5	130 ± 11
Westag97::RLK	Yes	nd		nd	5	12 ± 3
B. Line::gene	Fungus infested	Root mass (g)	Significant differences	Range (g)	n	Root RS	Leaf DS
X5	No	7.80	a	4.90–8.78	15	1.0 ± 0.1	3.2 ± 0.7
X5	Yes	3.86	bc	3.08–4.80	15	4.5 ± 0.9	4.3 ± 0.8
X5::RLK	No	6.10	a	4.77–7.81	15	1.0 ± 0.1	1.0 ± 0.3
X5::RLK	Yes	4.64	b	4.00–6.22	15	1.5 ± 0.5	1.5 ± 0.4
Westag97	Yes	6.15	A	3.02–9.55	6	6.5 ± 1.9	6.3 ± 1.3
Westag97::RLK	Yes	12.2	B	3.55–24.0	6	0.5 ± 0.3	1.8 ± 0.5
C. Line::gene	Insect PI (%)	Mean shoot dry weight (g)		n	Leaf defoliation (%)	Seed No	weight
X5	22 + 13	47.2	a	4	33 ± 4.0	40 ± 9	11.6 ± 0.3
X5::RLK	59 + 15	18.8	b	4	90 ± 5.0	36 ± 8	11.2 ± 0.3

Part A shows SCN female index in greenhouse grown seedlings at 28 days after SCN infestations. Pots were watered daily with 100 ml. Female index (FI) was the mean percentage of cysts of Hg Type 0 found on five plants per repetition compared to a susceptible genotype Essex. Part B shows the effects of the transgene on resistance to F. virguliforme. in greenhouse grown seedlings at 28 days after infestations. Pots were saturated with water to the 5 cm level. Leaf scorch was recorded as the mean disease severity (DS) measured on a 1–9 scale found on five plants per experimental repeat. Root rot severity (IS) was measured on a 1–5 scale. Panel C shows the percent insect incidence, defoliation by herbivorous insects and the consequent loss of biomass at harvest as mean dry weight per plant for field grown plants with 4 replications across 2 years.

ISSN: 1471-2164