Figure 1

Venn-diagrams of genes differentially expressed in cv. Dream after treatment and timepoint comparisons. (Sections A-C) Treatment comparison at 32 h after inoculation (hai) between 1,795 genes differentially expressed after ‘cv. Dream Fusarium inoculated versus cv. Lynx Fusarium inoculated’ and 1,700 genes differentially expressed after ‘cv. Dream mock inoculated versus cv. Lynx mock inoculated’. The Venn-diagram shows the numbers of differentially up- or down-regulated (+/−) genes that were assigned to the following categories of transcript occurrence: (Section A) FHB-responsive genes (656) were assumed to reflect induced cv. Dream-controlled differences between both cultivars as they were not differential expressed in the mock-inoculated controls of both cultivars and in the susceptible cv. Lynx after FHB treatment. (Section B) Genotype-specific genes (1,139) that were differentially expressed upon both treatments in cv. Dream, but not in cv. Lynx. (Section C) The remaining genes (561) were assumed to represent the genetic background of the Dream cultivar as they were also found to be differentially expressed in the absence of FHB-inoculation. For the timepoint 72 hai a corresponding categorisation could not be done due the low quality of the microarrays of the mock treated samples from the Lynx cultivar. (Sections D-F) Timepoint comparison for 1,795 genes differentially expressed after ‘cv. Dream Fusarium inoculated versus cv. Lynx Fusarium inoculated’ at 32 hai in reference to 1,737 genes differentially expressed after the analogous comparison at 72 hai. The Venn-diagram shows the numbers of differentially up- or down-regulated (+/−) genes at the certain timepoints: (Section D) Genes found to be differentially expressed in the cv. Dream exclusively at the timepoint 32 hai (752); (Section E) at both timepoints (1,043); and (Section F) exclusively at the timepoint 72 hai (694).