Figure 2From: Ultra-high resolution HLA genotyping and allele discovery by highly multiplexed cDNA amplicon pyrosequencingPositioning and sequence of HLA DRB universal amplification primers. An alignment of all known HLA DRB alleles produced a variability profile. (a) The depth of coverage (i.e. the number of alleles at each position in the alignment) is shown in grey, along with the percentage of nucleotide sequence variability (red) and the position of the DRB genotyping amplicon. (b) The sequence and composition of universal amplification primers for the DRB amplicon.Back to article page