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Table 2 Expression and secretome data of predicted glycosyl hydrolases

From: The carbon starvation response of Aspergillus niger during submerged cultivation: Insights from the transcriptome and secretome

     

Transcriptomic data

     
     

Expressiona

 

Fold changesb

 

FDR q-valuesb

 

Secretome datac

Identifier

Gene

CAZy

(Predicted) function

SPd

Expe

Day 1

Day 3

Day 6

 

Day 1

Day 3

Day 6

 

Day 1

Day 3

Day 6

 

Expe

Day 1

Day 3

Day 6

Chitin

An09g02240

nagA

GH20

β-1,6-N-acetylglucosaminidase

1

1.1

64

52

48

 

57.4

46.4

43.5

 

2.4E-12

5.0E-12

5.6E-12

 

-

++++

+++++

+++++

An02g07020

chiB

GH18

chitinase

0

1.6

73

77

84

 

45.0

47.2

51.5

 

3.9E-14

4.1E-14

3.4E-14

 

-

++

-

-

An01g05360

cfcD

GH18

chitinase

0

1.2

10

11

10

 

8.1

8.9

7.7

 

2.8E-12

1.9E-12

3.7E-12

 

-

-

-

-

An01g01920

 

GH20

β-1,6-N-acetylglucosaminidase

1

0.5

2

1

2

 

3.1

2.5

3.6

 

5.6E-08

9.3E-07

1.5E-08

 

-

-

+++

++

An02g02340

csmB

GT2

chitin synthase

0

7.7

19

25

22

 

2.5

3.2

2.9

 

1.8E-09

1.1E-10

2.9E-10

 

-

-

-

-

An09g02290

chsD

GT2

chitin synthase

0

4.4

10

11

10

 

2.3

2.6

2.3

 

5.1E-09

1.4E-09

8.0E-09

 

-

-

-

-

An09g04010

chsB

GT2

chitin synthase

0

7.5

16

16

17

 

2.1

2.1

2.2

 

4.1E-08

4.7E-08

1.9E-08

 

-

-

-

-

An08g05290

chsG

GT2

chitin synthase

1

0.5

1

1

0

 

1.6

1.3

1.0

 

1.6E-04

1.5E-02

7.5E-01

 

-

-

-

-

An02g02360

csmA

GT2

chitin synthase

0

2.9

5

6

4

 

1.6

2.0

1.5

 

2.1E-03

4.9E-05

6.5E-03

 

-

-

-

-

An02g13580

cfcI

GH18

chitinase

1

0.6

1

24

43

 

2.3

41.6

73.5

 

1.2E-02

3.9E-09

6.9E-10

 

-

-

-

-

An09g05920

ctcB

GH18

chitinase

1

0.5

1

16

42

 

1.3

33.8

90.2

 

1.2E-01

2.1E-11

1.2E-12

 

-

-

-

+++∗

α-glucan

An07g08640

agnB

GH71

α-glucanase

1

0.5

33

27

6

 

68.7

57.7

13.4

 

8.4E-15

1.3E-14

1.2E-12

 

-

+++

++

-

An15g04760

agnE

GH71

α-glucanase

1

0.4

0

2

1

 

1.0

3.6

1.7

 

7.2E-01

1.5E-08

1.6E-04

 

-

-

-

-

An09g03100

agtA

GH13

α-glucan transferase

1

6.2

14

15

18

 

2.3

2.4

2.8

 

3.2E-04

1.9E-04

4.0E-05

 

-

-

-

-

An02g03260

agsD

GH13/GT5

α-glucan synthase

1

0.4

0

1

1

 

1.0

2.3

1.5

 

9.5E-01

1.9E-06

1.4E-03

 

-

-

-

-

β-glucan

An01g03090

 

GH81

β-glucanase

1

1.1

34

56

57

 

29.8

49.3

50.5

 

1.1E-14

4.3E-15

3.7E-15

 

-

-

-

-

An02g13180

 

GH55

β-glucanase

1

0.4

4

2

3

 

11.1

6.1

8.1

 

5.4E-11

1.7E-09

2.9E-10

 

-

+++

+++∗

++∗

An01g04560

 

GH16

β-glucanase

1

1.2

10

23

31

 

8.2

19.8

26.5

 

1.4E-11

3.3E-13

1.1E-13

 

-

+++∗

++++

++++∗

An18g04100

 

GH28

β-glucanase

1

0.8

4

16

17

 

5.9

21.0

22.8

 

1.3E-07

2.5E-10

1.8E-10

 

-

-

-

-

An01g11010

crhD

GH16

β-glucanase

1

21.3

92

61

44

 

4.3

2.9

2.1

 

1.2E-09

6.3E-08

4.6E-06

 

+++∗

+++++

+++++

+++++

An07g04650

bgtC

GH17

β-glucanase

0

1.4

6

7

6

 

4.0

5.0

4.3

 

2.6E-09

5.3E-10

1.6E-09

 

+∗

-

-

-

An01g12450

bxgA

GH55

β-glucanase

1

7.5

26

23

34

 

3.5

3.0

4.5

 

6.4E-08

2.9E-07

7.6E-09

 

++∗

++++

+++++

+++++

An11g01540

 

GH16

β-glucanase

1

1.0

2

1

1

 

2.1

1.0

0.6

 

6.2E-06

9.9E-01

2.5E-04

 

++∗

++++

++++

++++∗

An10g00400

gelA

GH72

β-1,3-glucosyl transferase

1

12.6

20

52

50

 

1.6

4.1

4.0

 

4.3E-05

1.6E-10

2.1E-10

 

+++∗

++

-

-

An09g00670

gelD

GH72

β-1,3-glucosyl transferase

1

48.0

75

59

69

 

1.6

1.2

1.4

 

3.3E-05

1.5E-02

2.2E-04

 

+++

++++∗

+++++∗

+++++

An02g00850

 

GH16

β-glucanase

1

1.5

2

6

9

 

1.4

3.8

6.1

 

1.9E-03

1.3E-09

3.4E-11

 

-

-

-

++

An06g01550

fksA

GT48

β-1,3-glucan synthase

0

62.6

71

78

91

 

1.1

1.2

1.4

 

7.0E-02

2.6E-03

1.7E-05

 

-

-

-

-

An08g03580

bgtA

GH17

β-glucanase

1

0.7

1

1

2

 

1.1

1.9

2.8

 

4.3E-01

1.5E-05

9.9E-08

 

-

-

-

-

An02g09050

gelG

GH72

β-1,3-glucosyl transferase

1

0.6

1

2

6

 

1.0

3.6

9.0

 

9.3E-01

2.5E-06

4.6E-09

 

-

-

-

-

An16g02850

 

GH16

β-glucanase

1

1.4

1

5

3

 

0.8

3.6

2.3

 

1.3E-01

4.8E-09

7.7E-07

 

-

-

-

-

An06g01530

 

GH17

β-glucanase

1

0.6

1

1

3

 

0.8

2.3

4.4

 

2.5E-02

1.9E-07

2.3E-10

 

-

-

-

-

An02g03980

kslA

GH16

β-glucanase

0

0.5

0

1

2

 

0.8

2.4

4.7

 

4.2E-03

1.5E-08

2.0E-11

 

-

-

-

-

Mannan

An07g07700

 

GH76

α-1,6-mannanase

1

1.4

30

35

27

 

20.8

24.7

18.8

 

7.1E-13

4.5E-13

1.1E-12

 

-

+++

-

-

An01g06500

dfgD

GH76

α-1,6-mannanase

1

0.5

3

5

6

 

5.8

8.4

10.9

 

2.3E-10

2.9E-11

7.0E-12

 

-

-

-

-

An14g03520

dfgC

GH76

α-1,6-mannanase

1

3.6

7

6

7

 

1.9

1.8

1.9

 

1.1E-07

5.8E-07

1.9E-07

 

-

++∗

+++∗

-

An04g09650

 

GH76

α-1,6-mannanase

1

0.3

1

0

0

 

1.8

1.3

1.3

 

4.0E-05

3.4E-02

2.3E-02

 

-

+++∗

+++∗

+++

An02g02660

dfgG

GH76

α-1,6-mannanase

1

0.8

1

1

1

 

1.6

1.4

1.1

 

4.7E-03

3.5E-02

4.7E-01

 

-

-

-

-

An18g01410

dfgA

GH76

α-1,6-mannanase

1

0.7

1

1

2

 

0.8

1.5

2.7

 

2.4E-01

4.1E-03

1.9E-06

 

-

-

-

-

  1. amRNA abundance relative (%) to the gamma-actin (An15g00560) encoding transcript during exponential growth.
  2. bFold changes and FDR q-values for comparisons with transcriptome data from the exponential growth phas.
  3. cProtein abundance in filtrates: (-) not detected; (+) < 5 ng ml−1; (++) < 50 ng ml−1; (+++) < 250 ng ml−1; (++++) < 1 μ g ml−1; (+++++) < 4 μ g ml−1; (++++++) > 4 μ g ml−1; (∗) biological and/or technical relative standard deviation above 100 and 50, respectively.
  4. dSignal peptide sequence prediction[43].
  5. eExponential growth phase.