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Table 2 Expression and secretome data of predicted glycosyl hydrolases

From: The carbon starvation response of Aspergillus niger during submerged cultivation: Insights from the transcriptome and secretome

      Transcriptomic data      
      Expressiona   Fold changesb   FDR q-valuesb   Secretome datac
Identifier Gene CAZy (Predicted) function SPd Expe Day 1 Day 3 Day 6   Day 1 Day 3 Day 6   Day 1 Day 3 Day 6   Expe Day 1 Day 3 Day 6
Chitin
An09g02240 nagA GH20 β-1,6-N-acetylglucosaminidase 1 1.1 64 52 48   57.4 46.4 43.5   2.4E-12 5.0E-12 5.6E-12   - ++++ +++++ +++++
An02g07020 chiB GH18 chitinase 0 1.6 73 77 84   45.0 47.2 51.5   3.9E-14 4.1E-14 3.4E-14   - ++ - -
An01g05360 cfcD GH18 chitinase 0 1.2 10 11 10   8.1 8.9 7.7   2.8E-12 1.9E-12 3.7E-12   - - - -
An01g01920   GH20 β-1,6-N-acetylglucosaminidase 1 0.5 2 1 2   3.1 2.5 3.6   5.6E-08 9.3E-07 1.5E-08   - - +++ ++
An02g02340 csmB GT2 chitin synthase 0 7.7 19 25 22   2.5 3.2 2.9   1.8E-09 1.1E-10 2.9E-10   - - - -
An09g02290 chsD GT2 chitin synthase 0 4.4 10 11 10   2.3 2.6 2.3   5.1E-09 1.4E-09 8.0E-09   - - - -
An09g04010 chsB GT2 chitin synthase 0 7.5 16 16 17   2.1 2.1 2.2   4.1E-08 4.7E-08 1.9E-08   - - - -
An08g05290 chsG GT2 chitin synthase 1 0.5 1 1 0   1.6 1.3 1.0   1.6E-04 1.5E-02 7.5E-01   - - - -
An02g02360 csmA GT2 chitin synthase 0 2.9 5 6 4   1.6 2.0 1.5   2.1E-03 4.9E-05 6.5E-03   - - - -
An02g13580 cfcI GH18 chitinase 1 0.6 1 24 43   2.3 41.6 73.5   1.2E-02 3.9E-09 6.9E-10   - - - -
An09g05920 ctcB GH18 chitinase 1 0.5 1 16 42   1.3 33.8 90.2   1.2E-01 2.1E-11 1.2E-12   - - - +++
α-glucan
An07g08640 agnB GH71 α-glucanase 1 0.5 33 27 6   68.7 57.7 13.4   8.4E-15 1.3E-14 1.2E-12   - +++ ++ -
An15g04760 agnE GH71 α-glucanase 1 0.4 0 2 1   1.0 3.6 1.7   7.2E-01 1.5E-08 1.6E-04   - - - -
An09g03100 agtA GH13 α-glucan transferase 1 6.2 14 15 18   2.3 2.4 2.8   3.2E-04 1.9E-04 4.0E-05   - - - -
An02g03260 agsD GH13/GT5 α-glucan synthase 1 0.4 0 1 1   1.0 2.3 1.5   9.5E-01 1.9E-06 1.4E-03   - - - -
β-glucan
An01g03090   GH81 β-glucanase 1 1.1 34 56 57   29.8 49.3 50.5   1.1E-14 4.3E-15 3.7E-15   - - - -
An02g13180   GH55 β-glucanase 1 0.4 4 2 3   11.1 6.1 8.1   5.4E-11 1.7E-09 2.9E-10   - +++ +++ ++
An01g04560   GH16 β-glucanase 1 1.2 10 23 31   8.2 19.8 26.5   1.4E-11 3.3E-13 1.1E-13   - +++ ++++ ++++
An18g04100   GH28 β-glucanase 1 0.8 4 16 17   5.9 21.0 22.8   1.3E-07 2.5E-10 1.8E-10   - - - -
An01g11010 crhD GH16 β-glucanase 1 21.3 92 61 44   4.3 2.9 2.1   1.2E-09 6.3E-08 4.6E-06   +++ +++++ +++++ +++++
An07g04650 bgtC GH17 β-glucanase 0 1.4 6 7 6   4.0 5.0 4.3   2.6E-09 5.3E-10 1.6E-09   + - - -
An01g12450 bxgA GH55 β-glucanase 1 7.5 26 23 34   3.5 3.0 4.5   6.4E-08 2.9E-07 7.6E-09   ++ ++++ +++++ +++++
An11g01540   GH16 β-glucanase 1 1.0 2 1 1   2.1 1.0 0.6   6.2E-06 9.9E-01 2.5E-04   ++ ++++ ++++ ++++
An10g00400 gelA GH72 β-1,3-glucosyl transferase 1 12.6 20 52 50   1.6 4.1 4.0   4.3E-05 1.6E-10 2.1E-10   +++ ++ - -
An09g00670 gelD GH72 β-1,3-glucosyl transferase 1 48.0 75 59 69   1.6 1.2 1.4   3.3E-05 1.5E-02 2.2E-04   +++ ++++ +++++ +++++
An02g00850   GH16 β-glucanase 1 1.5 2 6 9   1.4 3.8 6.1   1.9E-03 1.3E-09 3.4E-11   - - - ++
An06g01550 fksA GT48 β-1,3-glucan synthase 0 62.6 71 78 91   1.1 1.2 1.4   7.0E-02 2.6E-03 1.7E-05   - - - -
An08g03580 bgtA GH17 β-glucanase 1 0.7 1 1 2   1.1 1.9 2.8   4.3E-01 1.5E-05 9.9E-08   - - - -
An02g09050 gelG GH72 β-1,3-glucosyl transferase 1 0.6 1 2 6   1.0 3.6 9.0   9.3E-01 2.5E-06 4.6E-09   - - - -
An16g02850   GH16 β-glucanase 1 1.4 1 5 3   0.8 3.6 2.3   1.3E-01 4.8E-09 7.7E-07   - - - -
An06g01530   GH17 β-glucanase 1 0.6 1 1 3   0.8 2.3 4.4   2.5E-02 1.9E-07 2.3E-10   - - - -
An02g03980 kslA GH16 β-glucanase 0 0.5 0 1 2   0.8 2.4 4.7   4.2E-03 1.5E-08 2.0E-11   - - - -
Mannan
An07g07700   GH76 α-1,6-mannanase 1 1.4 30 35 27   20.8 24.7 18.8   7.1E-13 4.5E-13 1.1E-12   - +++ - -
An01g06500 dfgD GH76 α-1,6-mannanase 1 0.5 3 5 6   5.8 8.4 10.9   2.3E-10 2.9E-11 7.0E-12   - - - -
An14g03520 dfgC GH76 α-1,6-mannanase 1 3.6 7 6 7   1.9 1.8 1.9   1.1E-07 5.8E-07 1.9E-07   - ++ +++ -
An04g09650   GH76 α-1,6-mannanase 1 0.3 1 0 0   1.8 1.3 1.3   4.0E-05 3.4E-02 2.3E-02   - +++ +++ +++
An02g02660 dfgG GH76 α-1,6-mannanase 1 0.8 1 1 1   1.6 1.4 1.1   4.7E-03 3.5E-02 4.7E-01   - - - -
An18g01410 dfgA GH76 α-1,6-mannanase 1 0.7 1 1 2   0.8 1.5 2.7   2.4E-01 4.1E-03 1.9E-06   - - - -
  1. amRNA abundance relative (%) to the gamma-actin (An15g00560) encoding transcript during exponential growth.
  2. bFold changes and FDR q-values for comparisons with transcriptome data from the exponential growth phas.
  3. cProtein abundance in filtrates: (-) not detected; (+) < 5 ng ml−1; (++) < 50 ng ml−1; (+++) < 250 ng ml−1; (++++) < 1 μ g ml−1; (+++++) < 4 μ g ml−1; (++++++) > 4 μ g ml−1; () biological and/or technical relative standard deviation above 100 and 50, respectively.
  4. dSignal peptide sequence prediction[43].
  5. eExponential growth phase.