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Table 3 Expression and secretome data of predicted protein hydrolases

From: The carbon starvation response of Aspergillus niger during submerged cultivation: Insights from the transcriptome and secretome

     Transcriptomic data  
     Expressiona   Fold changesb   FDR q-valuesb   Secretome datac
Identifier Gene (Predicted) function   Expd Day 1 Day 3 Day 6   Day 1 Day 3 Day 6   Day 1 Day 3 Day 6   Expd Day 1 Day 3 Day 6
S P e present                     
An01g00530 pepB A4 family peptidase   0.6 84.9 32.5 113.9   148.1 56.7 198.8   1.4E-16 9.1E-16 5.9E-17   - - - -
An14g04710 pepA Aspartyl protease   1.4 180.1 34.7 70.5   130.2 25.1 51.0   1.9E-13 2.2E-11 2.2E-12   +++ ++++++ ++++++ ++++++
An01g01750   Subtilisin-like serine protease   1.1 45.8 9.8 8.9   40.0 8.6 7.7   2.3E-14 9.5E-12 1.5E-11   - +++ ++++ +++
An02g01550   Secreted serine protease   2.4 63.2 25.3 6.0   26.0 10.4 2.5   8.6E-10 4.8E-08 9.8E-04   ++ ++++ ++++ -
An08g04640   Lysosomal pepstatininsensitive protease   1.1 16.2 8.0 10.6   14.3 7.1 9.3   4.0E-12 1.5E-10 3.2E-11   + ++++ +++++ ++++
An06g00190   Lysosomal pepstatininsensitive protease   2.3 31.5 27.2 25.9   13.4 11.6 11.0   1.1E-10 2.3E-10 2.8E-10   +++ ++++ +++++ ++++
An03g01010   Lysosomal pepstatininsensitive protease   1.2 14.9 3.2 4.2   12.7 2.7 3.6   1.2E-09 4.8E-05 3.6E-06   + +++ +++ +++
An02g04690   Serine-typecarboxypeptidase I   6.4 64.7 31.7 34.9   10.1 4.9 5.4   9.7E-10 8.4E-08 4.2E-08   ++ ++++ +++++ +++++
An12g05960   Dipeptidyl peptidase II   1.0 9.2 4.1 2.7   9.0 4.0 2.6   2.8E-11 7.3E-09 4.78-07   - +++ ++++ ++++
An07g08030 pepF Serine carboxypeptidase   0.8 7.0 4.2 6.3   8.6 5.2 7.7   1.2E-11 2.7E-10 2.2E-11   - ++ +++ +++
An11g06350   Carboxypeptidase   1.1 8.3 3.6 1.7   7.9 3.4 1.6   5.0E-11 2.1E-08 5.0E-04   - - - -
An09g03780 pepD Subtilisin-like serineprotease   0.5 3.0 0.6 0.7   5.8 1.1 1.4   2.2E-11 1.6E-01 2.4E-03   - - - -
An12g03300   Aspartic protease   0.7 3.4 0.5 0.4   5.0 0.7 0.7   1.1E-08 1.2E-02 1.0E-02   ++ +++   
An15g06280   Aspartic proteinase[truncated ORF]   5.8 25.2 33.2 22.4   4.3 5.7 3.8   4.1E-09 6.1E-10 1.2E-08   ++ +++ ++++ ++++
An16g09010   Carboxypeptidase I[putative frameshift]   0.6 2.2 3.6 4.7   4.0 6.4 8.4   2.5E-09 8.8E-11 1.8E-11   - - +++ +++
An14g00620   Aminopeptidase   5.3 20.4 14.7 14.3   3.8 2.8 2.7   4.6E-09 1.3E-07 1.8E-07   - - - -
An07g03880 pepC Serine proteinase   31.1 111.2 101.5 91.5   3.6 3.3 2.9   8.5E-12 2.2E-11 6.3E-11   ++ - - -
An07g10060   Proteinase B inhibitor   4.2 13.1 17.0 19.5   3.1 4.1 4.7   1.1E-08 1.1E-09 3.5E-10   +++ - - -
An02g07210 pepE Aspartic protease   30.4 83.9 57.8 57.2   2.8 1.9 1.9   1.4E-10 3.3E-08 3.9E-08   - ++++ +++ ++
An15g07700   Aspergillopepsin IIprecursor   1.3 3.5 3.3 4.4   2.7 2.6 3.4   3.5E-07 5.2E-07 3.1E-08   - - - -
An18g01320   Extracellular proteaseprecursor   21.9 54.8 14.7 4.9   2.5 0.7 0.2   1.8E-05 1.5E-02 6.8E-08   +++ ++++ +++++ ++++
An02g13740   Gly-X carboxypeptidaseprecursor   1.9 4.4 4.2 4.4   2.3 2.2 2.4   9.5E-09 2.1E-08 8.6E-09   - - - -
An03g01660   Vacuolar aminopeptidase Y   9.1 19.2 17.4 17.5   2.1 1.9 1.9   3.1E-09 1.9E-08 1.5E-08   ++ - - -
An08g08750 cpY Carboxypeptidase   33.8 67.9 53.8 51.3   2.0 1.6 1.5   1.0E-08 1.3E-06 4.2E-06   ++ - - -
An14g03250   Aspergillopepsin II   0.7 1.2 0.9 1.6   1.7 1.3 2.2   2.5E-04 3.0E-02 3.1E-06   - +++ - -
An07g10410   Metalloprotease   1.2 1.3 7.5 16.1   1.1 6.5 13.9   2.8E-01 8.3E-11 1.6E-12   - - - -
S P e absent                     
An01g00370   Aspergillopepsin   0.8 89.0 16.8 10.0   110.2 20.8 12.3   3.6E-14 4.9E-12 3.9E-11   ++ +++++ +++++ +++++
An02g00090   Prolidase   0.4 20.4 22.7 7.6   53.2 59.4 19.8   1.2E-14 1.0E-14 1.9E-13   - - - -
An14g02080   Prolidase   1.8 18.0 6.8 4.1   9.7 3.7 2.2   4.4E-09 2.8E-06 3.8E-04   - - - -
An09g02830   Acylaminoacyl-peptidase   4.3 39.7 18.0 9.9   9.2 4.2 2.3   1.8E-12 3.0E-10 1.6E-07   - - - -
An17g00390   Aminopeptidase   4.6 27.7 10.2 7.4   6.0 2.2 1.6   3.2E-11 5.1E-07 1.2E-04   - - - -
An18g03980   Glutamatecarboxypeptidase II   3.2 19.0 13.8 13.7   5.9 4.3 4.3   3.3E-12 3.3E-11 3.4E-11   - - - -
An01g01720   Bleomycin hydrolase   0.6 3.3 1.4 1.0   5.4 2.3 1.7   1.6E-10 8.4E-07 1.0E-04   - - - -
An07g06490   Insulin-degrading enzyme   0.4 2.1 1.1 1.1   5.2 2.7 2.7   8.5E-11 2.9E-08 3.7E-08   - - - -
An11g05920   Prolidase   0.9 4.6 1.5 1.3   5.0 1.6 1.4   5.2E-08 9.1E-03 5.3E-02   - - - -
An11g02950   Calpain family cysteine protease   0.9 3.6 3.2 1.7   4.3 3.7 2.0   9.8E-10 3.4E-09 6.0E-06   - - - -
An01g14920   Metallopeptidase   0.5 1.8 1.3 1.1   3.9 2.8 2.3   1.1E-09 4.0E-08 4.1E-07   - - - -
An11g01970   Pyroglutamyl peptidase   2.1 8.2 6.0 6.8   3.9 2.8 3.2   1.0E-11 2.4E-10 6.2E-11   - - - -
An14g01530   Subtilisin-like serine proteases   0.5 2.0 1.1 0.7   3.7 2.1 1.3   9.9E-09 7.0E-06 2.6E-02   - - - -
An09g06800   Leucyl aminopeptidase   10.9 40.3 37.7 33.4   3.7 3.5 3.1   1.3E-11 2.5E-11 8.2E-11   - - - -
An12g01820   Ubiquitin carboxyl-terminal hydrolase   0.8 2.6 1.7 1.1   3.4 2.2 1.5   8.1E-10 2.0E-07 4.4E-04   - - - -
An01g08470   Ubiquitin carboxyl-terminal hydrolase   1.8 5.9 7.5 7.4   3.3 4.2 4.1   6.1E-10 8.3E-11 9.6E-11   - - - -
An04g00410   Dipeptidyl peptidase III   17.0 51.9 37.8 29.6   3.1 2.2 1.7   9.6E-10 5.6E-08 3.4E-06   - - - -
An16g08150   Dipeptidyl-peptidase V   10.5 26.0 17.4 17.2   2.5 1.7 1.6   4.3E-09 4.1E-06 4.9E-06   - - - -
An18g02980   Endopeptidase   4.6 10.4 8.3 8.2   2.3 1.8 1.8   4.0E-09 2.0E-07 2.6E-07   - - - -
An04g06940 prtT Transcriptional activator of proteases   5.4 58.4 35.2 24.9   10.8 6.5 4.6   5.7E-12 9.8E-11 1.0E-09   - - - -
  1. amRNA abundance relative (%) to the gamma-actin (An15g00560) encoding transcript during exponential growth.
  2. bFold changes and FDR q-values for comparisons with transcriptome data from the exponential growth phase.
  3. cProtein abundance in filtrates: (-) not detected; (+) < 5 ng ml−1; (++) < 50 ng ml−1; (+++) < 250 ng ml−1; (++++) < 1 μ g ml−1; (+++++) < 4 μ g ml−1; (++++++) > 4 μ g ml−1; () biological and/or technical relative standard deviation above 100 and 50, respectively.
  4. dExponential growth phase.
  5. eSignal peptide sequence prediction[43].
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