Analysis of plant-derived miRNAs in animal small RNA datasets

Zhang, Yuanji; Wiggins, B Elizabeth; Lawrence, Christina; Petrick, Jay; Ivashuta, Sergey; Heck, Greg

doi:10.1186/1471-2164-13-381

BMC Genomics

Table 2 Sequencing result and reads mapped to plant miRNAs from insect and plant sRNA libraries in Run 1

From: Analysis of plant-derived miRNAs in animal small RNA datasets

Sample ID	Organism	Description	Raw reads	plant miRNA	%plant/all**	top plant miRNA	reads	miR168 reads (rank)
lettuce 1 ~ 10*	Lettuce	10 individual lettuce samples	151549912	975866	97.558	396	589392	70430 (2 ~ 4)
diet 1	Corn	leaf	15015721	368664	98.911	396	95687	8952 (9)
diet 2	Soybean	leaf	17723129	1775205	99.918	159	446258	13467 (15)
diet 3	Corn	root	9890019	71668	82.096	156	29703	4533 (4)
feeding 1	WCR	neonate	16544248	1045	0.099	1507	207	19 (10)
feeding 2	CEW	neonate	14016629	790	0.073	1507	205	41 (6)
feeding 3	FAW	neonate	15429925	859	0.061	1507	403	12 (7)
feeding 4	CEW	diet corn leaf, carcass 5^th instar rep1	13469808	165	0.012	1507	55	1 (14)
feeding 5	CEW	diet corn leaf, carcass 5^th instar rep2	19188383	872	0.418	1507	329	9 (10)
feeding 6	CEW	diet corn leaf, carcass 5^th instar rep3	13020144	210	0.019	168	43	43 (1)
feeding 7	FAW	diet corn leaf, carcass 5^th instar rep1	14496854	648	0.077	1507	162	32 (7)
feeding 8	FAW	diet corn leaf, carcass 5^th instar rep2	23688055	592	0.017	1507	182	13 (8)
feeding 9	FAW	diet corn leaf, carcass 5^th instar rep3	8346959	305	0.028	1507	143	2 (14)
feeding 10	FAW	diet soy leaf, carcass 5^th instar rep1	13583831	903	0.229	1507	263	27 (9)
feeding 11	FAW	diet soy leaf, carcass 5^th instar rep2	20007178	1065	0.310	1507	231	33 (8)
feeding 12	FAW	diet soy leaf, carcass 5^th instar rep3	16090620	2773	0.298	159	719	28 (12)
feeding 13	WCR	diet corn root, carcass 3^rd instar rep1	23799773	509	0.064	1507	177	10 (8)
feeding 14	WCR	diet corn root, carcass 3^rd instar rep2	20263174	400	0.051	1507	134	6 (10)
feeding 15	WCR	diet corn root, carcass 3^rd instar rep3	18023806	242	0.038	1507	70	7 (7)
feeding 16	CEW	diet soy leaf, carcass 5^th instar rep1	13292508	2947	0.361	3522	671	12 (14)
feeding 17	CEW	diet soy leaf, carcass 5^th instar rep2	17506112	1744	0.110	3522	574	13 (13)
feeding 18	CEW	diet soy leaf, carcass 5^th instar rep3	failed

*Numbers in the row are the totals of 10 lettuce libraries.
**This is the ratio of plant miRNA reads over all miRNA reads. For plant datasets all miRNAs include contaminated animal miRNAs.

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ISSN: 1471-2164

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