Sequencing of LPK Nek8 mutation. Chromographs show the lpk mutation as a SNP within the Nek8 gene. Lines A and B are the Brown Norway (BN) reference sequence (REF) (NC_005109.2, gene ID: 287473) and the BN parent sequence data; both showed a homozygous Cytosine (C) at the position of the SNP (red box). Lines C and D are the LPK parent and BC1 (1: cystic positive) progeny and contained the homozygous Thymidine (T). Panel E is the BC1 (0: cystic negative) progeny and is heterozygous (C/T). Panel F is the other parental rat strain Wistar Kyoto (WKY) which had a homozygous Cytosine (C) at the position of the SNP.