Location of Nek8 mutation and species sequence alignment. Sequence alignment of Nek8 protein from 7 representative species, created from known Nek8 mRNA sequences with the MAFFT algorithm . Panel A illustrates the complete Nek8 alignment. Black indicates 100% conservation scaled to white (0% conservation). The location of the R650C mutation is highlighted within the red box. The serine/threonine kinase region (N terminal) shows high conservation while the alpha tubulin suppressor domain region of the protein (C terminal) shows less conservation and contains the regulator of chromosome condensation-1 (RCC1) domains. Panel B shows the area of the R650C mutation in greater detail to illustrate the conservation of the G[R]LG across the species. The R650C lies within a RCC1 domain. Species represented are the Rattus (rat), Danio (zebra fish), Gallus Gallus (rooster), Xenopus (frog), Homo (human), Mus muscularis (mouse) and Saccoglossus (acorn worm) and their respective accession numbers.