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Figure 2 | BMC Genomics

Figure 2

From: Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica

Figure 2

Analysis of imprinting at the MEST locus in M. domestica. ( A ) 3′ RACE distinguished two alternative MEST transcripts. The vertical arrows indicate the polymorphic sites used to analyze the imprint status of the two transcripts. ( B ) Transcript 1 (n = 2) is imprinted since the genomic A/G polymorphism is absent in the cDNA, demonstrating that only one allele is expressed. The parental origin of expression for transcript 1 could not be determined because a heterozygous offspring with a mother homozygous at the A/G polymorphic site was not found. In contrast, transcript 2 (n = 1) is not imprinted since the C/A polymorphism is present in both the genomic DNA and the cDNA.

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