Figure 3
From: Convergent and divergent evolution of genomic imprinting in the marsupial Monodelphis domestica
Methylation profiles of M. domestica CpG-rich regions close to orthologues of eutherian imprinted genes. Bisulfite-modified genomic DNA was PCR amplified and cloned. Each line denotes an individual cloned allele. Representative CpG-rich regions analyzed are ( A ) putative promoter region of HTR2A, ( B ) 5′ upstream region of L3MBTL, ( C ) 5′ upstream region of MEST, ( D ) 5′ upstream region of IMPACT, ( E ) 5′ upstream region of COPG2, and ( F ) putative promoter region of IGF2R. Unfilled circles depict unmethylated cytosines at CpG sites, while filled circles depict methylated cytosines. The genomic co-ordinates of these regions are listed in Additional file1: Table S3.