Figure 2

A representative image of DNA fingerprints of the positive BAC clones for determination of overlapping relationship. The positive BAC clones identified in the previous steps were digested with Hin d III, followed by separation on a 1% agarose gel in 1× TAE buffer. The gel was stained with Ethidium Bromide (EB) for photograph with a UVP Labworks system. M: Marker of DNA size standard (1 kb plus DNA ladder from Invitrogen, San Diego, CA, USA) with the base pair (bp) sizes indicated on both sides.