Table 1 Genes analyzed by quantitative real-time PCR
| Â | Gene | Primer sequence | Size (bp) | Accession number (GenBank) |
|---|---|---|---|---|
Upregulated genes/sequences | APP | F: ACCCCTGACGCCGTTGAC | 121 | NM_001076796.1 |
R: TCATGACCTGGGACATCCTCTC | ||||
AQP4 | F: GTTCACGGAAATCTTAGCGCT | 104 | NM_181003.2 | |
R: TCAGTCCGTTTGGAATCACAG | ||||
CPPED1 | F: TTGGATGGCATCACCGACTT | 101 | NM_001031771.2 | |
R: TTTGCGACCTCATGAACCAC | ||||
GOLGA4 | F: TCTACCAAAACCACTGCCTCAA | 88 | NM_001192125.1 | |
R: TCCCACTACTGGCTCTACATCACT | ||||
MPP7 | F: GCCTCCTATGCCTGATGACAT | 81 | NM_001100347.1 | |
R: CCCAGTGGTTCTCTATTTTTGACC | ||||
NELL2 | F: AAGAGGGAGACGATGGACTGAG | 105 | NM_001102084.1 | |
R: ACACCAAGACCCCAAACTGCT | ||||
OSRS1* | F: GAGGATCTTGTGGAACCATTGA | 124 | FQ482089.2 | |
R: TACGGACAGCTGAACCCTTTC | ||||
OSRS2** | F: TTGTCAGTCCCCATCACCTTT | 101 | NW_003104406.1 | |
R: CATTGATTTGCACAGAAAACCA | ||||
Downregulated genes | CD3G | F: AGCTTCAGACAAGCAGACGCT | 101 | BC103010.1 |
R: GGGTTCAGTTCTTCCTCAGGTG | ||||
GNLY | F: TCCGTGCCAGTCAATCATGA | 101 | NM_001075143.1 | |
R: TGCAGACCTTGATGTCCACAC | ||||
LAPTM4B | F: GGTACTTGATCCTCAATGCCG | 101 | NM_205802.1 | |
R: AAAGTCACCCCCGAGTTCAGA | ||||
SRSF3 | F: CGAAATGCATCGTGATTCCT | 101 | NM_001034700.1 | |
R: AATAGCCAAAAGCTCGTTCCA |